Poly(beta malic acid) with pendant leu-leu-leu tripeptide for effective cytoplasmic drug delivery
The invention relates to the use of Polycefin-LLL nanoconjugate as a means of cytoplasmic delivery of drugs. In one embodiment, the present invention provides a drug delivery molecule, comprising a polymerized carboxylic acid molecular scaffold covalently linked to L-leucylleucylleucine. In another embodiment, the Polycefin-LLL includes drug antisense morpholino oligos, targeting antibodies, and a pH-sensitive endosome escape unit. In addition, the drug could be siRNA, microRNA, and aptamer.
1. A drug delivery system, comprising: a polymerized polymalic acid molecular scaffold having a plurality of pendant carboxyl groups and one or more biologically active molecular modules, each of the one or more biologically active molecular module is covalently linked to a pendant carboxyl group of the polymerized polymalic acid molecular scaffold, wherein one of the one or more biologically active molecular modules comprises an endosome escape unit, the endosome escape unit comprises L-leucylleucylleucine (LLL).
2. The drug delivery system of claim 1 , wherein the endosome escape unit is configured to cause disruption of 60%, or more of endosome membranes at a pH of 5.5, or lower.
3. The drug delivery system of claim 1 , wherein one of the one or more biologically active molecular modules comprises a siRNA, a microRNA, and/or an aptamer.
4. The drug delivery system of claim 1 , wherein L-leucylleucylleucine (LLL) is conjugated to 10% or more of the pendant carboxyl groups of the polymerized polymalic acid molecular scaffold.
5. The drug delivery system of claim 1 , wherein L-leucylleucylleucine (LLL) is conjugated to 40% or more of the pendant carboxyl groups of the polymerized polymalic acid molecular scaffold.