Methods, compounds, and compositions for treatment and prophylaxis in the respiratory tract
The present invention provides a method of reducing the quanitity of mucus in the respiratory tract of a subject with elevated levels of mucus in said respiratory tract. The method includes administering to the subject a compound or composition containing a therapeutically effective amount of a fusion protein comprising a sialidase or an active portion thereof and an anchoring domain. The therapeutically effective amount comprises an amount of the fusion protein that results in a reduction of the quanitity of mucus in the respiratory tract after administration of the compound or composition when compared to the quantity of mucus present prior to administration of the compound or composition.
1. A method of limiting an increase in the quantity of mucus in the respiratory tract of a subject above a baseline level of mucus in the subject's respiratory tract, comprising:
determining a baseline level of mucus in the subject's respiratory tract; and
administering to the subject a compound or composition containing a therapeutically effective amount of a fusion protein, wherein
the fusion protein comprises at least one catalytic domain of a sialidase, wherein the catalytic domain of the sialidase comprises the sequence of amino acids extending from amino acid 274 to amino acid 666 of SEQ ID NO:12, inclusive, and at least one anchoring domain, wherein the anchoring domain is a glycosaminoglycan (GAG) binding domain of human amphiregulin comprising the amino acid sequence of SEQ ID NO:7, wherein
the therapeutically effective amount comprises an amount of the fusion protein that limits an increase in the quantity of mucus in the respiratory tract of the subject above the baseline level.
2. The method of claim 1 , wherein the composition further comprises one or more additional compounds.
3. The method of claim 2 , wherein the one or more additional compounds comprises MgSO 4 .
4. The method of claim 2 , wherein the one or more additional compounds comprises CaCl 2 .
5. The method of claim 2 , wherein the one or more additional compounds comprises Histidine.
6. The method of claim 2 , wherein the one or more additional compounds comprises Histidine-HCl.
7. The method of claim 2 , wherein the one or more additional compounds comprises Trehalose.
8. The method of claim 2 , wherein the one or more additional compounds comprises MgSO 4 , CaCl 2 , Histidine, Histine-HCl and Trehalose.
9. The method of claim 1 , wherein the compound or composition is formulated as a spray.
10. The method of claim 1 , wherein the compound or composition is formulated as an inhalant.
11. The method of claim 1 , wherein the compound or composition is formulated as a solution for injection.
12. The method of claim 1 , wherein the compound or composition is formulated as a cream, salve, gel, or ointment.
13. The method of claim 1 , wherein the compound or composition is formulated as a pill, tablet, lozenge, suspension, or solution that can be administered orally.
14. The method of claim 1 , wherein said administration is by use of a nasal spray.
15. The method of claim 1 , wherein said administration is by use of an inhaler.
16. The method of claim 1 , wherein said administration is performed from once to four times a day.
17. The method of claim 1 , wherein said administration is by topical application.
18. The method of claim 1 , wherein said administration is by oral administration.
19. The method of claim 1 , wherein said administration is by local or intravenous injection.
20. The method of claim 1 , wherein the subject is human.
21. The method of claim 1 , wherein the subject is a non-human animal.
22. The method of claim 1 , wherein the subject has one or more of the following conditions: chronic obstructive pulmonary disease (COPD), bronchitis, bronchiectasis, cystic fibrosis (CF), vasculitis, mucus plugging, Wegener's granulomatosis, pneumonia, tuberculosis, cancer involving the lungs or the respiratory tract, Kartagener syndrome, Young's syndrome, chronic sinopulmonary infection, alpha 1-antitrypsin deficiency, primary immunodeficiency, acquired immune deficiency syndrome, opportunistic infection, an infectious state, a post infectious state, common cold, exercise induced hypersecretion of mucus, inflammatory bowel disease, ulcerative colitis, Crohn's disease, respiratory infection, respiratory obstruction, inhalation or aspiration of a toxic gas, pulmonary aspiration, or alcoholism.
23. The method of claim 1 , wherein the fusion protein has the sequence set forth in SEQ ID NO:21.
24. A method of limiting an increase in the quantity of mucus in the respiratory tract of a subject above a baseline level of mucus in the subject's respiratory tract, comprising:
determining a baseline level of mucus in the subject's respiratory tract; and
administering to the subject a compound or composition containing a therapeutically effective amount of a fusion protein comprising a sialidase or an active portion thereof and an anchoring domain, wherein
the therapeutically effective amount comprises an amount of the fusion protein that limits an increase in the quantity of mucus in the respiratory tract of the subject above the baseline level.
25. The method of claim 24 , wherein said sialidase or active portion thereof is substantially homologous to Actinomyces viscosus sialidase or its catalytic domain.
26. The method of claim 24 , wherein said anchoring domain is a glycosaminoglycan (GAG) binding domain.
27. The method of claim 24 , wherein the composition further comprises one or more additional compounds.
28. The method of claim 27 , wherein the one or more additional compounds comprises MgSO 4 .
29. The method of claim 27 , wherein the one or more additional compounds comprises CaCl 2 .
30. The method of claim 27 , wherein the one or more additional compounds comprises Histidine.
31. The method of claim 27 , wherein the one or more additional compounds comprises Histidine-HCl.
32. The method of claim 27 , wherein the one or more additional compounds comprises Trehalose.
33. The method of claim 27 , wherein the one or more additional compounds comprises MgSO 4 , CaCl 2 , Histidine, Histine-HCl and Trehalose.
34. The method of claim 24 , wherein the compound or composition is formulated as a spray.
35. The method of claim 24 , wherein the compound or composition is formulated as an inhalant.
36. The method of claim 24 , wherein the compound or composition is formulated as a solution for injection.
37. The method of claim 24 , wherein the compound or composition is formulated as a cream, salve, gel, or ointment.
38. The method of claim 24 , wherein the compound or composition is formulated as a pill, tablet, lozenge, suspension, or solution that can be administered orally.
39. The method of claim 24 , wherein said administration is by use of a nasal spray.
40. The method of claim 24 , wherein said administration is by use of an inhaler.
41. The method of claim 24 , wherein said administration is performed from once to four times a day.
42. The method of claim 24 , wherein said administration is by topical application.
43. The method of claim 24 , wherein said administration is by oral administration.
44. The method of claim 24 , wherein said administration is by local or intravenous injection.
45. The method of claim 24 , wherein the subject is a human.
46. The method of claim 24 , wherein the subject is a non-human animal.
47. The method of claim 24 , wherein the subject has one or more of the following conditions: chronic obstructive pulmonary disease (COPD), bronchitis, bronchiectasis, cystic fibrosis (CF), vasculitis, mucus plugging, Wegener's granulomatosis, pneumonia, tuberculosis, cancer involving the lungs or the respiratory tract, Kartagener syndrome, Young's syndrome, chronic sinopulmonary infection, alpha 1-antitrypsin deficiency, primary immunodeficiency, acquired immune deficiency syndrome, opportunistic infection, an infectious state, a post infectious state, common cold, exercise induced hypersecretion of mucus, inflammatory bowel disease, ulcerative colitis, Crohn's disease, respiratory infection, respiratory obstruction, inhalation or aspiration of a toxic gas, pulmonary aspiration, or alcoholism.
48. The method of claim 24 , wherein the fusion protein has the sequence set forth in SEQ ID NO:21.
49. The method of any one of claims 1 and 24 , further comprising selecting a subject for treatment.
50. The method of claim 49 , wherein the subject is not infected with one or more of influenza virus, parainfluenza virus, and/or respiratory syncytial virus (RSV).
51. The method of claim 49 , wherein the subject is infected with one or more of influenza virus, parainfluenza virus, and/or RSV.
52. The method of claim 49 , wherein the subject has asthma.
53. The method of claim 50 , wherein the subject has asthma.
54. The method of claim 51 , wherein the subject further has asthma.
55. The method of claim 51 , wherein the compound or composition comprises a protein or peptide having the sequence set forth in SEQ ID NO:21.
56. The method of claim 1 or 24 , further comprising determining a second level of mucus in the subject's respiratory tract prior to administering to the subject the compound or composition.
57. The method of claim 56 , further comprising continuing treatment until the level or increase in level of mucus in the subject's respiratory tract is decreased or limited in the second level of mucus compared to the baseline level of mucus in the subject's respiratory tract.