Polypeptides having xylanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A nucleic acid construct or recombinant expression vector comprising an isolated polynucleotide encoding a polypeptide having xylanase activity, wherein the polynucleotide is operably linked to one or more heteroloqous control sequences that direct the production of the polypeptide in an expression host, wherein the polypeptide having xylanase activity is selected from the group consisting of:
(a) a polypeptide having at least 95% sequence identity to the sequence of amino acids 19 to 414 of SEQ ID NO: 4;
(b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) nucleotides 55 to 1242 of SEQ ID NO: 3 or (ii) a full-length complementary strand of (i), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, followed by three washing steps each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; and
(c) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to the sequence of nucleotides 55 to 1242 of SEQ ID NO: 3.
2. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide has at least 96% sequence identity to the sequence of amino acids 19 to 414 of SEQ ID NO: 4.
3. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide has at least 97% sequence identity to the sequence of amino acids 19 to 414 of SEQ ID NO: 4.
4. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide has at least 98% sequence identity to the sequence of amino acids 19 to 414 of SEQ ID NO: 4.
5. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide has at least 99% sequence identity to the sequence of amino acids 19 to 414 of SEQ ID NO: 4.
6. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide is a fragment of the amino acid sequence of SEQ ID NO: 4 having xylanase activity.
7. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide comprises the sequence of SEQ ID NO: 4.
8. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide comprises the sequence of amino acids 19 to 414 of SEQ ID NO: 4.
9. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with (i) nucleotides 55 to 1242 of SEQ ID NO: 3 or (ii) a full-length complementary strand of (i), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, followed by three washing steps each for 15minutes using 2×SSC, 0.2% SDS at 65° C.
10. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 55 to 1242 of SEQ ID NO: 3 or (ii) a full-length complementary strand of (i) wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, followed by three washing steps each for 15minutes using 2×SSC, 0.2% SDS at 70° C.
11. An isolated recombinant host cell comprising the DNA construct or recombinant expression vector of claim 1 .
12. A method of producing a polypeptide having xylanase activity, said method comprising:
(a) cultivating the recombinant host cell of claim 11 under conditions conducive for production of the polypeptide: and
(b) recovering the polypeptide.