Apoptosis modulator Bcl-B and methods for making and using same
View Patent ↗A novel human member of the Bcl-2 family Bcl-B has been identified, which is closest in amino-acid sequence homology to the Boo (Diva) protein. The Bcl-B protein is widely expressed in adult human tissues. The Bcl-B protein modulates apoptosis. Bcl-B also binds Bcl-2, BCl-XL, and Bax but not Bak. Bcl-B displays a unique pattern of selectivity for binding and regulating the function of other members of the Bcl-2 family.
1. An isolated or recombinant polypeptide comprising a sequence having at least about 99% identity to SEQ ID NO:2.
2. An isolated or recombinant polypeptide comprising at least 200 amino acids of SEQ ID NO:2.
3. The isolated or recombinant polypeptide of claim 1 , wherein the polypeptide contains a transmembrane domain.
4. The isolated or recombinant polypeptide of claim 1 , wherein the transmembrane domain comprises a mitochondrial protein or a Bcl-2 protein family member transmembrane domain.
5. The isolated or recombinant polypeptide of claim 1 , wherein the polypeptide has an activity selected from modulating apoptosis, homodimerization, heteromerization, binding to Bcl-2, Bcl-XL or Bax, forming a membrane channel, associating with mitochondria, and immunogenicity.
6. The isolated or recombinant polypeptide of claim 5 , wherein the polypeptide modulates apoptosis in a bacterial, plant, insect, mammalian or yeast cell.
7. The isolated or recombinant polypeptide of claim 6 , wherein the mammalian cell is a human cell selected from heart, brain, lung, kidney, liver, pancreas, spleen, thymus, colon, leukocyte, small intestine, testis, prostate and ovarian cell.
8. The isolated or recombinant polypeptide of claim 1 , wherein the polypeptide inhibits Bax mediated apoptosis.
9. The isolated or recombinant polypeptide of claim 1 , wherein the polypeptide does not detectably inhibit Bak mediated apoptosis.
10. The isolated or recombinant polypeptide of claim 1 , wherein the polypeptide contains one or more BH1, BH2, BH3 or BH4 domains.
11. The isolated or recombinant polypeptide of claim 1 , wherein the polypeptide is attached to a substrate.
12. A composition comprising a plurality of polypeptides attached to a substrate, wherein one or more of the polypeptide comprises the isolated or recombinant polypeptide of claim 1 .
13. The composition of claim 12 , wherein the polypeptides are attached at defined positions of the substrate.
14. An isolated or recombinant polypeptide comprising a sequence having 100% identity to SEQ ID NO:2.
15. The isolated or recombinant polypeptide of claim 14 , wherein the polypeptide contains a transmembrane domain.
16. The isolated or recombinant polypeptide of claim 14 , wherein the transmembrane domain comprises a mitochondrial protein or a Bcl-2 protein family member transmembrane domain.
17. The isolated or recombinant polypeptide of claim 14 , wherein the polypeptide has an activity selected from modulating apoptosis, homodimerization, heteromerization, binding to Bcl-2, Bcl-XL or Bax, forming a membrane channel, associating with mitochondria, and immunogenicity.
18. The isolated or recombinant polypeptide of claim 17 , wherein the polypeptide modulates apoptosis in a bacterial, plant, insect, mammalian or yeast cell.
19. The isolated or recombinant polypeptide of claim 18 , wherein the mammalian cell is a human cell selected from heart, brain, lung, kidney, liver, pancreas, spleen, thymus, colon, leukocyte, small intestine, testis, prostate and ovarian cell.
20. The isolated or recombinant polypeptide of claim 14 , wherein the polypeptide inhibits Bax mediated apoptosis.
21. The isolated or recombinant polypeptide of claim 14 , wherein the polypeptide does not detectably inhibit Bak mediated apoptosis.
22. The isolated or recombinant polypeptide of claim 14 , wherein the polypeptide contains one or more BH1, BH2, BH3 or BH4 domains.
23. The isolated or recombinant polypeptide of claim 14 , wherein the polypeptide is attached to a substrate.
24. A composition comprising a plurality of polypeptides attached to a substrate, wherein one or more of the polypeptide comprises the isolated or recombinant polypeptide of claim 14 .
25. The composition of claim 24 , wherein the polypeptides are attached at defined positions of the substrate.