IP Library Granted Patent US 8,765,983
Granted Patent B2
US 8,765,983 · App. 12/983,767 · Granted Jul 1, 2014

Systems and methods for extracting lipids from and dehydrating wet algal biomass

Inventors: Daniel Fleischer (Oakland, CA); Marko Jukic (San Francisco, CA); Andrew Thompson (Oakland, CA); Guido Radaelli (Oakland, CA)
Assignee: Aurora Algae, Inc.
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 8,765,983
App. No.
12/983,767
Granted
Jul 1, 2014
Kind
B2
Abstract

Exemplary methods include centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass, mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture, heating the mixture to result in a dehydrated, defatted algal biomass, separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids, evaporating the amphiphilic solvent from the water and the lipids, and separating the water from the lipids. The amphiphilic solvent may be selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. Other exemplary methods include filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass.

Claims (30)

1. A method comprising:

mixing algal biomass with an amphiphilic solvent;

separating the amphiphilic solvent from algal solids, or from any part of the algal biomass not dissolved in the amphiphilic solvent, to result in amphiphilic solvent, water and lipids;

evaporating most or substantially all of the amphiphilic solvent from the water and the lipids, to result in a mixture of the water and the lipids; and

separating the lipids from the mixture.

2. The method of claim 1 , wherein the amphiphilic solvent is selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde.

3. A method comprising:

filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass;

mixing the filtered algal biomass with an amphiphilic solvent to result in a mixture;

heating the mixture to result in a dehydrated, defatted algal biomass;

separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids;

evaporating the amphiphilic solvent from the water and the lipids; and

separating the water from the lipids.

4. The method of claim 3 , wherein the wet algal biomass is filtered to increase the solid content to approximately 30%.

5. The method of claim 3 , wherein the amphiphilic solvent is selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde.

6. The method of claim 3 , wherein the mixture is heated in a pressurized reactor.

7. The method of claim 6 , wherein the pressurized reactor is a batch or a continuous pressurized reactor.

8. The method of claim 3 , wherein the mixture is heated with microwaves, ultrasound, steam, or hot oil.

9. The method of claim 3 , wherein the amphiphilic solvent is separated from the dehydrated, defatted algal biomass via membrane filtration to result in amphiphilic solvent, water and lipids.

10. The method of claim 3 , wherein the amphiphilic solvent is separated from the dehydrated, defatted algal biomass via centrifugation to result in amphiphilic solvent, water and lipids.

11. The method of claim 3 , wherein the separating includes decanting the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids.

12. The method of claim 3 , wherein the separating of the water from the lipids includes adding a nonpolar solvent.

13. The method of claim 12 , wherein the nonpolar solvent is propane, butane, pentane, hexane, butene, propene, naphtha or gasoline.

14. The method of claim 3 , wherein the separating of the water from the lipids includes decanting the lipids without a nonpolar solvent.

15. The method of claim 3 , wherein the separating of the water from the lipids includes adding a nonpolar solvent in a continuous liquid-liquid extractor.

16. The method of claim 15 , wherein the nonpolar solvent is evaporated from the lipids by distillation or flash evaporation.

17. The method of claim 3 , wherein the separating of the water from the lipids includes adding a nonpolar solvent in a batch vessel and decanting the batch vessel.

18. The method of claim 17 , wherein the nonpolar solvent is evaporated from the lipids by distillation or flash evaporation.

19. The method of claim 3 , wherein the wet algal biomass is centrifuged to increase the solid content to approximately 30%.

20. The method of claim 3 , wherein the evaporating the amphiphilic solvent from the water and the lipids is performed by flash evaporation, distillation or by pervaporation.

Assignments (3)
RELEASE OF SECURITY INTEREST Recorded Apr 20, 2015
From: SILICON VALLEY BANK, AS AGENT
To: AURORA ALGAE, INC.
Reel/Frame 035452/0305 →
SECURITY INTEREST Recorded Nov 8, 2011
From: AURORA ALGAE, INC.
To: SILICON VALLEY BANK
Reel/Frame 027249/0001 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 19, 2011
From: FLEISCHER, DANIEL; JUKIC, MARKO; THOMPSON, ANDY; RADAELLI, GUIDO
To: AURORA ALGAE, INC.
Reel/Frame 026312/0834 →
Continuity (2)
Continuation In Part 12610134 · Oct 30, 2009
Related Publication 20110196163A1 · Aug 11, 2011