Tissue expander
Provided herein is a tissue stretching device including a tissue clamping member defining an area in a Z plane, wherein the tissue clamping member is configured to hold tissue parallel to the Z plane. Methods of use of the tissue stretching device to stretch a tissue as well as for culturing organized tissues are also provided. Stretched and/or cultured tissues produced by these processes are also provided, as well as methods of treatment making use of the same.
1. A tissue stretching device comprising:
a tissue clamping member comprising from 14 to 28 clamps and defining an area in a Z plane, wherein said tissue clamping member is configured to hold tissue parallel to said Z plane, wherein said tissue clamping member has four sides along the Z plane and is configured to expand in area along the Z plane; wherein said clamps form a rectangular or square tissue clamping member
a displacement sensor operatively associated with said tissue clamping member, said displacement sensor configured to measure a linear displacement along an axis of the Z plane to thereby detect an amount of expansion of the tissue by said tissue clamping member; and
a controller operatively associated with said tissue clamping member, wherein said controller operates an actuator connected to said tissue clamping member to thereby stretch said tissue along the Z plane, wherein said clamps are slidably mounted on two pairs of oppositely facing support rods, wherein one of the pairs of oppositely facing support rods is fixed, and the other of the pairs of oppositely facing support rods is operatively connected to a pair of drive assemblies, wherein said actuator comprises a pair of actuators in which the drive assemblies are operably connected to the pair of actuators.
2. The device of claim 1 , wherein said controller is operatively associated with a force sensor, whereby said force sensor senses the force applied to the tissue in said tissue clamping member upon the application of force by said actuator connected thereto.
3. The device of claim 1 , wherein said tissue clamping member is enclosed in a chamber configured to hold media, wherein said media is in fluid contact with said tissue clamping member.
4. The device of claim 3 further comprising a media reservoir, and wherein said chamber comprises a media inlet and media outlet, said media inlet being in fluid communication with said media reservoir.
5. The device of claim 3 wherein said chamber further comprises a gas inlet configured to provide a gas to the tissue.
6. The device of claim 1 , wherein said tissue clamping member comprises a first set of adjoining sides comprising a first plurality of clamps and a second set of adjoining sides comprising a second plurality of clamps, and wherein the first set of adjoining sides are fixed relative to said chamber, and the second set of adjoining sides are movable along said Z plane.
7. The device of claim 1 , wherein the device is configured to stretch skin tissue.
8. The device of claim 1 , wherein the device is configured to stretch muscle tissue.
9. A method of stretching a tissue comprising:
clamping the tissue into the device of claim 1 ; and
stretching the tissue with the device to thereby stretch the tissue.
10. The method of claim 9 , wherein the tissue is skin tissue or muscle tissue.
11. A method of treating a skin wound of a subject in need thereof, comprising:
providing a skin tissue;
stretching the skin tissue with the device of claim 1 to form a stretched skin tissue; and then
grafting the stretched skin tissue onto said subject, to thereby treat the skin wound.
12. The method of claim 11 , wherein the skin tissue is allogeneic.
13. The method of claim 11 , wherein the skin tissue is autologous.
14. A method of culturing organized tissue comprising:
(a) providing cells on a support, wherein said support is clamped into the device of claim 1 ; then
(b) cyclically stretching and relaxing said support at least twice along a first (X) axis during a first time period;
(c) cyclically stretching and relaxing said support at least twice along a second (Z) axis during said first time period; and then
(d) maintaining said support in a substantially static position during a second time period; and then
(e) repeating steps (b) through (d) for a number of times sufficient to enhance the functionality of the tissue or to produce organized tissue on said solid support from said cells.
15. The method of claim 14 , wherein said cyclically stretching and relaxing along said first axis, along said second axis, or along both said first and second axis is carried out at least three times during said first time period.
16. The method of claim 14 , wherein said stretching along said first axis, along said second axis, or along both said first and second axis comprises extending said support to a dimension at least 5% greater in length than said static position.
17. The method of claim 14 , wherein said relaxing along said first axis, along said second axis, or along both said first and second axis comprises retracting said support to a dimension not greater in length than said static position.
18. The method of claim 14 , wherein said relaxing along said first axis, along said second axis, or along both said first and second axis comprises retracting said support to a dimension at least 5% lesser in length than said static position.
19. The method of claim 14 , wherein said first time period is from 2 to 30 minutes in duration.
20. The method of claim 14 , wherein said second time period is from 10 to 100 minutes in duration.
21. The method of claim 14 , wherein said repeating of steps (b) through (d) is carried out for at least one week.
22. The device of claim 1 , wherein upon retraction of the pair of drive assemblies by the pair of actuators, the clamps become spaced apart, and upon reversing the direction of the pair of actuators, the clamps group closer together, until individual clamps contact one another.