Reagent for measurement of active oxygen
A compound represented by the formula (I) (R 1 represents an aryl group such as hydroxyphenyl group, R 2 represents 2-carboxyphenyl group etc., and R 3 and R 4 represent —(CH 2 ) p —N(R 5 )(R 6 ) (R 5 and R 6 represent —(CH 2 ) n —COOH), a salt thereof, or an ester thereof, which is useful as a reagent for highly sensitive measurement of reactive oxygen such as hydroxy radical present at a trace amount in living bodies or tissues by a bioimaging technique over a long period of time.
1. A compound represented by the following formula (I):
wherein R 1 represents an aryl group which may be substituted, R 2 represents a 2-carboxyphenyl group which may be substituted, and R 3 and R 4 independently represent a group represented as —(CH 2 ) p —N(R 5 )(R 6 ) (in the formula, p represents an integer of 1 to 4, and R 5 and R 6 independently represent —(CH 2 ) n —COOH (in the formula, n represents an integer of 1 to 4)), a salt thereof, or an ester thereof.
2. The compound, a salt thereof, or an ester thereof according to claim 1 , wherein R 1 is p-aminophenyl group or p-hydroxyphenyl group.
3. The compound, a salt thereof, or an ester thereof according to claim 1 , wherein R 2 is 2-carboxyphenyl group.
4. The compound, a salt thereof, or an ester thereof according to claim 1 , wherein R 3 and R 4 represent —(CH 2 )—N[(CH 2 )—COOH] 2 .
5. An ester of the compound according to claim 1 , which is a tetracetoxymethyl ester formed with R 3 or R 4 .
6. A reagent for measurement of reactive oxygen, which contains the compound, a salt thereof, or an ester thereof according to claim 1 .
7. The reagent according to claim 6 , wherein the reactive oxygen species is hydroxyl radical, peroxynitrite, or hypochlorous acid.
8. The compound, a salt thereof, or an ester thereof according to claim 2 , wherein R 2 is 2-carboxyphenyl group.
9. The compound, a salt thereof, or an ester thereof according to claim 2 , wherein R 3 and R 4 represent —(CH 2 )—N[(CH 2 )—COOH] 2 .
10. The compound, a salt thereof, or an ester thereof according to claim 3 , wherein R 3 and R 4 represent —(CH 2 )—N[(CH 2 )—COOH] 2 .
11. An ester of the compound according to claim 2 , which is a tetracetoxymethyl ester formed with R 3 or R 4 .
12. An ester of the compound according to claim 3 , which is a tetracetoxymethyl ester formed with R 3 or R 4 .
13. An ester of the compound according to claim 4 , which is a tetracetoxymethyl ester formed with R 3 or R 4 .
14. A reagent for measurement of reactive oxygen, which contains the compound, a salt thereof, or an ester thereof according to claim 2 .
15. A reagent for measurement of reactive oxygen, which contains the compound, a salt thereof, or an ester thereof according to claim 3 .
16. A reagent for measurement of reactive oxygen, which contains the compound, a salt thereof, or an ester thereof according to claim 4 .
17. A reagent for measurement of reactive oxygen, which contains the compound, a salt thereof, or an ester thereof according to claim 5 .
18. A reagent for measurement of reactive oxygen, which contains the compound, a salt thereof, or an ester thereof according to claim 8 .
19. A reagent for measurement of reactive oxygen, which contains the compound, a salt thereof, or an ester thereof according to claim 9 .
20. A reagent for measurement of reactive oxygen, which contains the compound, a salt thereof, or an ester thereof according to claim 10 .