Mesenchymal stem cells for the treatment of CNS diseases
An isolated human cell is disclosed comprising at least one mesenchymal stem cell phenotype and secreting brain-derived neurotrophic factor (BDNF), wherein a basal secretion of the BDNF is at least five times greater than a basal secretion of the BDNF in a mesenchymal stem cell. Methods of generating same and uses of same are also disclosed.
1. An isolated, non-genetically modified human cell differentiated from a bone marrow mesenchymal stem cell expressing at least one bone marrow derived mesenchymal stem cell marker, not secreting nerve growth factor (bNGF) and secreting brain-derived neurotrophic factor (BDNF), wherein a basal secretion of said BDNF is at least five times greater than a basal secretion of said BDNF in a non-differentiated, non-genetically modified mesenchymal stem cell, wherein said at least one bone marrow derived mesenchymal stem cell marker is selected from the group consisting of CD7, CD90 and CD105.
2. The isolated human cell of claim 1 , which takes up at least ten times more glutamate from its surroundings than said non-differentiated, non-genetically modified mesenchymal stem cell.
3. The isolated human cell of claim 1 , being star-shaped.
4. The isolated human cell of claim 1 , further expressing GFAP.
5. The isolated human cell of claim 1 , further expressing GDNF.
6. The isolated human cell of claim 1 , wherein said BDNF is secreted at a concentration of about 5782-8452 pg/10 6 cells.