System and method for focusing optics
In an apparatus and system for focusing optics an objective lens is configured to collect light from a region of an object to be imaged, said region having a feature with a known geometric characteristic, wherein the geometric characteristic is known before the feature is imaged by the optical device. A focusing sensor is configured to observe a shape of the feature and a splitter is configured to split the collected light into a first portion and a second portion, and directing said first portion through a weak cylindrical lens to the focusing sensor. A processor is configured to analyze the observed shape and determine whether the observed shape of the feature has a predetermined relationship to the known geometric characteristic and a mechanism is configured to autofocus the optical device by moving at least one of the objective lens and the object to be imaged in response to the analysis and determination of the processor. In some embodiments, the feature can be a fluorescent bead. In some embodiments, the splitting step can be accomplished with a dichroic mirror. In other embodiments, the splitting step can be accomplished with a partial mirror. In some embodiments, the known geometric characteristic of the feature can be substantially spherical, the observed shape can be an oval, and the predetermined relationship can be an allowable aspect ratio of the oval. In some embodiments, the allowable aspect ratio can be approximately one.
1. An apparatus for focusing an optical device, comprising:
an objective lens configured to collect light from a region of an object to be imaged, said region having a feature with a known geometric characteristic, wherein the geometric characteristic is known before the feature is imaged by the optical device;
a focusing sensor configured to observe a geometric characteristic of the feature;
a splitter configured to split the collected light into a first portion and a second portion, and directing said first portion through a weak cylindrical lens to the focusing sensor;
a processor configured to analyze the observed geometric characteristic and determine, by comparing the observed geometric characteristic to the known geometric characteristic, a predetermined relationship between the observed geometric characteristic and the known geometric characteristic; and
a mechanism configured to autofocus the optical device by moving at least one of the objective lens and the object to be imaged based on the predetermined relationship, in response to the analysis and determination of the processor.
2. The apparatus of claim 1 , wherein the collected light is at least one of:
light reflecting from the region as a result of incident light from a laser source; and
light emitted from a fluorescent bead.
3. The apparatus of claim 1 , wherein the optical device is a microscope.
4. The apparatus of claim 1 , wherein the splitter is a dichroic mirror.
5. The apparatus of claim 1 , wherein the splitter is a partial mirror.
6. The apparatus of claim 1 , wherein the known geometric characteristic is the feature being substantially spherical, wherein the observed shape is an oval, and wherein the predetermined relationship is an allowable aspect ratio of the oval.
7. The apparatus of claim 6 , wherein the allowable aspect ratio is approximately one.
8. The apparatus of claim 1 , further comprising an imager, wherein the splitter directs the second portion of the collected light to the imager and wherein at least one of the focusing sensor and the imager produce a digital image.
9. The apparatus of claim 8 , wherein the digital image is captured using at least one of a CMOS chip and a CCD chip.
10. The apparatus of claim 8 , wherein the digital image is compared to a stored digital image to determine whether the observed shape of the feature has the predetermined relationship to the known geometric characteristic.
11. The apparatus of claim 10 , wherein the comparison is performed using at least one of the processor and a field-programmable gate array (FPGA).
12. The apparatus of claim 1 , wherein the mechanism comprises at least one of a motor and a piezoelectric device.
13. The apparatus of claim 12 , wherein the processor is coupled to the mechanism and the processor is adapted to control the mechanism.
14. The apparatus of claim 13 , wherein the processor directs the mechanism to move at least one of the objective lens and an object to be imaged until the observed shape has the predetermined relationship to the known geometric characteristic.
15. The apparatus of claim 14 , wherein the processor predicts an appropriate final position of at least one of the objective lens and the object to be imaged prior to directing the mechanism.
16. A system for focusing an optical device, comprising:
an objective lens configured to collect light from a region of an object to be imaged, said region having a feature with a known geometric characteristic, wherein the geometric characteristic is known before the feature is imaged by the optical device;
a focusing sensor configured to observe a geometric characteristic of the feature;
a light splitter configured to split the collected light into a first portion and a second portion, and directing said first portion through a weak cylindrical lens to the focusing sensor, wherein the focusing sensor observes a geometric characteristic of the feature;
a processor, coupled to the motor and focusing sensor, configured to analyze the observed geometric characteristic and determine, by comparing the observed shape to the known geometric characteristic, a predetermined relationship between the observed geometric characteristic and the known geometric characteristic; and
a mechanism for autofocusing the optical device by moving at least one of the objective lens and the object to be imaged based on the predetermined relationship, in response to the analysis and determination of the processor.
17. The system of claim 16 , further comprising an imager, wherein the splitter directs the second portion of the collected light to the imager and wherein at least one of the focusing sensor and the imager produce a digital image.
18. The system of claim 17 , wherein the digital image is captured using at least one of a CMOS chip and a CCD chip.
19. The system of claim 17 , wherein the digital image is compared to a stored digital image to determine whether the observed shape of the feature has the predetermined relationship to the known geometric characteristic.
20. The system of claim 19 , wherein the comparison is performed using at least one of the processor and a field-programmable gate array (FPGA).
21. The system of claim 16 , wherein the mechanism is at least one of a motor and a piezoelectric device.