Biomarkers for lung disease monitoring
View Patent ↗The present invention pertains to the monitoring and treatment of lung transplant recipients. In particular, the invention pertains to the use of biomarkers to predict or detect post-lung transplantation complications (e.g., organ rejection, acute organ rejection, organ injury, bronchiolitis obliterans, bronchiolitis obliterans syndrome, organizing pneumonia), fibroproliferative repair responses, interstitial lung diseases (e.g., idiopathic pulmonary fibrosis and other fibrotic lung diseases), and other immune-mediated lung diseases (e.g., graft versus host disease, scleroderma).
1. A method of assessing the risk of bronchiolitis obliterans syndrome in a post lung transplant subject, comprising:
a) obtaining a sample of bronchoalveolar lavage fluid from a post lung transplant subject;
b) quantitating the number of lung-resident mesenchymal stem cells in said sample bronchoalveolar lavage fluid; wherein said quantitating comprises isolating nucleated cells from said sample and culturing said isolated cells to generate single separated fibroblastoid colonies and
c) determining an increased risk of bronchiolitis obliterans syndrome in said subject when a level of single separated fibroblast colonies of at least 10 colonies per 2×10 6 of isolated nucleated cells is present in said sample bronchoalveolar lavage fluid.
2. The method of claim 1 , wherein said subject is human.
3. The method of claim 1 , wherein said post lung transplant subject received a transplanted lung at least 3 month prior to the obtaining of said sample of bronchoalveolar lavage fluid.