Genes for enhanced lipid metabolism for accumulation of lipids
Provided herein are exemplary genes, constructs and methods for the formation of triacylglycerols (TAGs). The exemplary genes include a phosphatic acid phosphohydrolase (PA Hydrolase) gene, a diacylglycerol o-acyltransferase (DAGAT2A) gene, and a phospholipid:diacylglycerol acyltransferase (LROI) gene.
1. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequence set forth in SEQ. ID. NO. 1.
2. The method of claim 1 , wherein a promoter in an expression vector increases expression of the nucleotide sequence compared to a wild-type algal cell.
3. The method of claim 1 , wherein the algal cell is of algal genus Nannochloropsis.
4. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequence set forth in SEQ. ID. NO. 2.
5. The method of claim 4 , wherein a promoter in an expression vector increases expression of the nucleotide sequence compared to a wild-type algal cell.
6. The method of claim 4 , wherein the algal cell is of algal genus Nannochloropsis.
7. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequence set forth in SEQ. ID. NO. 3.
8. The method of claim 7 , wherein a promoter in an expression vector increases expression of the nucleotide sequence compared to a wild-type algal cell.
9. The method of claim 7 , wherein the algal cell is of algal genus Nannochloropsis.
10. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequences set forth in SEQ. ID. NO. 1 and SEQ. ID. NO. 2.
11. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequences set forth in SEQ. ID. NO. 1 and SEQ. ID. NO. 3.
12. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequences set forth in SEQ. ID. NO. 2 and SEQ. ID. NO. 3.
13. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequences set forth in SEQ. ID. NO. 1, SEQ. ID. NO. 2, and SEQ. ID. NO. 3.