Taxane- and taxoid-protein compositions
Compositions comprising a taxane or taxoid, such as paclitaxel, and a protein, such as albumin or a metal-transferrin, such as gallium-transferrin, can be prepared by combining an aqueous protein solution with a second solution containing the taxane or taxoid, a non-ionic surfactant, and an alcohol; adjusting the pH of the combined solutions to between about 7.9 and about 8.3; and purifying the pH-adjusted solution to remove solutes having a molecular weight less than 10,000 Da. Such compositions can be prepared that are substantially free of non-ionic surfactants, such as Cremophor EL, and are useful in the treatment of cancers, such as breast cancer.
1. A composition comprising A) a complex consisting of (i) a metal-transferrin; and (ii) a taxane or taxoid that inhibits mitosis; and B) a pharmaceutically acceptable diluent,
wherein the taxane or taxoid either (a) is bonded with the metal-transferrin via a direct bond between the taxane or taxoid and the metal-transferrin; or (b) forms a non-covalent complex with the metal-transferrin;
the transferrin of the metal-transferrin is human transferrin, bovine transferrin, serotranferrin, lactotransferrin, ovotransferrin, or melanotransferrin, and
the composition substantially lacks solutes having a molecular weight less than 10,000 Da.
2. The composition of claim 1 , wherein the taxane or taxoid is paclitaxel or docetaxel.
3. The composition of claim 1 , the metal-transferrin is gallium-transferrin, iron-transferrin, indium-transferrin, zinc-transferrin, manganese-transferrin, platinum-transferrin, or a mixture thereof.
4. The composition of claim 3 , the metal-transferrin is gallium-transferrin.
5. The composition of claim 1 , wherein the taxane or taxoid is paclitaxel.
6. The composition of claim 5 , the metal-transferrin is gallium-transferrin.
7. The composition claim 1 , wherein the taxane or taxoid is bonded with the metal-transferrin via a direct bond.
8. The composition of claim 1 , wherein the taxane or taxoid forms a non-covalent complex with the metal-transferrin.