Method and devices for improved oxygen permeability in microorganism storage containers
The various embodiments disclosed herein relate to improved containers for transporting microorganisms and other living material. Certain embodiments include air-permeable bladders having an air-permeable inner wall and a stronger outer wall having perforations.
1. An oxygen-permeable bladder comprising two walls coupled to each other along each outer edge of the two walls, wherein each of the two walls comprises:
(a) a first film defining an inner wall of the bladder, the first film comprising a thin flexible film permeable to oxygen and carbon dioxide and impermeable to liquid; and
(b) a second film disposed adjacent to an outer surface of the first film and comprising a plurality of perforations,
wherein the first and second films are only bonded to each other along each outer edge of the two walls, and wherein each of the plurality of perforations has a diameter ranging from about 0.1 mm to about 3 mm, and wherein one of the two walls comprises a spout extending from the one of the two walls, the spout defining an opening in fluid communication with an inner cavity of the bladder.
2. The oxygen-permeable bladder of claim 1 , wherein the first film has an oxygen permeability of at least 5,500 cc/m 2 /day.
3. The oxygen-permeable bladder of claim 1 , further comprising a cover configured to be coupleable to the spout.
4. The oxygen-permeable bladder of claim 3 , wherein the first film has a thickness ranging from about 15 μm to about 90 μm.
5. The oxygen-permeable bladder of claim 3 , wherein the first film comprises polyethylene or polypropylene.
6. The oxygen-permeable bladder of claim 3 , wherein the second film has a thickness ranging from about 40 μm to about 80 μm.
7. The oxygen-permeable bladder of claim 3 , wherein the second film comprises polyester, polyethylene, polypropylene, or polyamide.
8. The oxygen-permeable bladder of claim 1 , wherein the first film has a thickness ranging from about 15 μm to about 90 μm.
9. The oxygen-permeable bladder of claim 1 , wherein the first film comprises polyethylene or polypropylene.
10. The oxygen-permeable bladder of claim 1 , wherein the second film has a thickness ranging from about 40 μm to about 80 μm.
11. The oxygen-permeable bladder of claim 1 , wherein the second film comprises polyester, polyethylene, polypropylene, or polyamide.
12. A container for transporting live microorganisms, the container comprising: (a) a substantially rigid external container; and (b) an oxygen-permeable bladder configured to be disposed within the substantially rigid external container, the bladder comprising (i) an inner film comprising a flexible film permeable to oxygen and carbon dioxide and impermeable to liquid and having a thickness ranging from about 15 μm to about 100 μm; and (ii) an outer film adjacent to the inner film, the outer film comprising a plurality of perforations, wherein the outer film is bonded to the inner film solely along four outer edges of the outer film, and wherein each of the plurality of perforations has a diameter ranging from about 0.1 mm to about 3 mm.
13. The container of claim 12 , wherein the inner film has an oxygen permeability of at least 5,500 cc/m 2 /day.
14. The container of claim 12 , wherein the bladder further comprises a spout associated with the bladder.