Trichoderma genes
Described herein are novel gene sequences isolated from Trichoderma reesei . Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
1. An isolated polynucleotide encoding a protein having cellulose binding activity which is:
(a) a nucleic acid sequence which encodes or is complementary to a sequence which encodes a CIP1 polypeptide having at least 90% sequence identity to the amino acid sequence presented as SEQ ID NO:5;
(b) a nucleic acid sequence which encodes or is complementary to a sequence which encodes a CIP1 polypeptide having at least 95% sequence identity to the amino acid sequence presented as SEQ ID NO:5;
(c) a nucleic acid sequence which encodes or is complementary to a sequence which encodes a CIP1 polypeptide having at least 98% sequence identity to the amino acid sequence presented as SEQ ID NO:5;
(d) a nucleic acid sequence which encodes or is complementary to a sequence which encodes a CIP1 polypeptide having the amino acid sequence presented as SEQ ID NO:5;
(e) a nucleic acid sequence which encodes or is complementary to a sequence which encodes a CIP1 polypeptide having at least 95% sequence identity to the amino acid sequence presented as SEQ ID NO:3;
(f) a nucleic acid sequence which encodes or is complementary to a sequence which encodes a CIP1 polypeptide having at least 98% sequence identity to the amino acid sequence presented as SEQ ID NO:3;
(g) a nucleic acid sequence which encodes or is complementary to a sequence which encodes a CIP1 polypeptide having the amino acid sequence presented as SEQ ID NO:3;
(h) a nucleic acid sequence presented as SEQ ID NO:2, or the complement thereof, wherein % identity is calculated using the CLUSTAL-W program in MacVector version 6.5, operated with default parameters, including an open gap penalty of 10.0, an extended gap penalty of 0.1, and a BLOSUM 30 similarity matrix; or
(i) a nucleic acid sequence that hybridizes, under high stringency conditions to the sequence presented as SEQ ID NO:2, or the complement or a fragment thereof, wherein hybridization is conducted at 42° C. in 50% formamide, 6×SSC, 5×Denhardt's solution, 0.5% SDS and 100 μg/ml denatured carrier DNA followed by washing two times in 2×SSPE and 0.5% SDS at room temperature and two additional times in 0.1 SSPE and 0.5% SDS at 42° C.
2. The isolated polynucleotide of claim 1 , wherein the enzyme is derived from a Trichoderma source.
3. The isolated polynucleotide of claim 2 , wherein the enzyme is derived from Trichoderma reesei.
4. An expression construct comprising the isolated polynucleotide of claim 1 .
5. A vector comprising the expression construct of claim 4 .
6. A vector comprising the isolated polynucleotide of claim 1 , operably linked to control sequences recognized by a host cell transformed with the vector.
7. A host cell transformed with the vector of claim 5 .
8. A host cell transformed with the vector of claim 6 .
9. The host cell of claim 7 , which is a prokaryotic cell.
10. The host cell of claim 8 , which is a eukaryotic cell.
11. A recombinant host cell comprising the isolated polynucleotide of claim 1 .
12. The recombinant host cell of claim 11 , which is a prokaryotic cell.
13. The isolated polynucleotide of claim 1 , wherein said polynucleotide is an RNA.
14. A method of producing an enzyme having cellulose binding activity comprising cultivating the host cell of claim 7 under condition suitable for the host cell to produce the enzyme, and recovering the enzyme.
15. A method of producing an enzyme having cellulose binding activity comprising cultivating the host cell of claim 8 under condition suitable for the host cell to produce the enzyme, and recovering the enzyme.
16. The method of claim 14 , wherein the host cell is a filamentous fungi or yeast cell.
17. The method of claim 15 , wherein the host cell is a filamentous fungi or yeast cell.