Polynucleotides encoding recombinant lubricin molecules and uses thereof
Recombinant lubricin molecules and uses thereof. Novel recombinant lubricin molecules and their uses as lubricants, anti-adhesive agents and/or intra-articular supplements for, e.g., synovial joints, meniscus, tendon, peritoneum, pericardium and pleura, are provided.
1. An isolated polynucleotide comprising a nucleic acid sequence encoding the polypeptide comprising the amino acid sequence of SEQ ID NO: 7.
2. An isolated polynucleotide, wherein the polynucleotide comprises the nucleic acid sequence of SEQ ID NO: 6.
3. The isolated polynucleotide of claim 1 comprising a nucleic acid sequence having at least 99% identity to the nucleic acid sequence of SEQ ID NO: 6.
4. An expression vector comprising the polynucleotide of claim 1 .
5. An expression vector of comprising the polynucleotide of claim 2 .
6. A host cell comprising the expression vector of claim 4 .
7. A host cell comprising the expression vector of claim 5 .
8. The host cell of claim 6 , wherein the cell is a mammalian cell.
9. The host cell of claim 7 , wherein the cell is a mammalian cell.
10. A method of producing a recombinant lubricin protein comprising:
a) growing the cell of claim 8 in culture medium; and
b) collecting the recombinant protein from the medium.
11. A method of producing a recombinant lubricin protein comprising:
a) growing the cell of claim 9 in culture medium; and
b) collecting the recombinant protein from the medium.