Synthesis of ultrabright fluorescent silica particles
The invention generally relates to fluorescent particles and more specifically to silica-based fluorescent nanoporous particles with physically encapsulated organic dyes. In one aspect of the invention, the nanoporous architecture provides a significant enhancement in fluorescence of the particles brightness compared to free dye. A particular chemical control of the silica matrix prevents the dye molecules from leaking the particles.
1. A method for synthesizing luminescent silica particles, the method comprising the steps of:
initiating a templating synthesizing reaction configured to synthesize a plurality of nanoporous silica particles, the templating synthesizing reaction including a first template and an acidic or basic catalyst, combined with a second solution comprising water, an organosilicate or sodium silicate as a first silica precursor, and a plurality of luminophore molecules; and
stirring a second silica precursor into the templating synthesizing reaction, wherein the second silica precursor is a triethoxysilane, wherein the luminophore molecules are encapsulated within a plurality of pores of said nanoporous silica particles, and further wherein said triethoxysilane prevents leakage of said luminophore molecules from said pores;
wherein the the first silica precursor comprises disodium trioxosilicate (Na 2 SiO 3 .9H 2 O), the first template comprises cetyltrimethylammonium chloride (CTAC), the catalyst comprises hydrochloric acid, the luminophore molecule comprises Rhodamine 6G (R6G), and the second silica precursor comprises ethyltriethoxysilane (ETES), wherein the molar ratio is: 0.13 Na 2 SiO 3 .9H 2 O:100 H 2 O:X R6G:0.21 CTAC:3.9 HCl:0.0063 ETES, where X is in the range from 3·10 −3 to 9·10 −3 .
2. The method of claim 1 wherein said nanoporous silica particles have sizes ranging from 10 nm to one micron in size.
3. The method of claim 1 wherein said nanoporous silica particles have sizes greater than one micron in size.