Nucleic acid modifying enzymes
This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
1. A polypeptide comprising at least two heterologous domains, wherein a first domain is a thermally stable sequence non-specific double-stranded nucleic acid binding domain and the second domain is a thermally stable polymerase domain that has polymerase activity, wherein the first domain specifically binds to polyclonal antibodies generated against either Sso7d (SEQ ID NO:2) or Sac7d (amino acids 7-71 of SEQ ID NO:10).
2. The polypeptide of claim 1 , wherein the polymerase domain is from a family A polymerase.
3. The polypeptide of claim 1 , wherein the polymerase domain is from a family B polymerase.
4. The polypeptide of claim 1 , wherein the polymerase domain is from a Thermus sp. polymerase.
5. The polypeptide of claim 1 , wherein the polymerase domain is from a Pyrococcus sp. polymerase.