Microchip and process for producing microchip
A microchip which comprises: a resinous base having a plurality of fine channels formed on one side thereof, one or more cylindrical parts disposed so as to protrude from the other side, and a through-hole which pierces each cylindrical part along the axis thereof and communicates with the fine channel so that the diameter of the inner wall of the through-hole gradually decreases from the tip end of the cylindrical part toward the fine channel at a first inclination angle; and a resinous covering member bonded to that side of the resinous base on which the fine channels have been formed. The microchip has been configured so that a liquid sample can be introduced from the tip end of each cylindrical part through the through-hole. The wall thickness of the cylindrical part on the end side where a liquid sample is to be introduced has been made smaller than the wall thickness thereof on the base side where the cylindrical part has been formed, by forming a step therebetween.
1. A microchip comprising:
a resinous base having at least one cylindrical part protruding from a surface of a substrate, a plurality of fine channels being formed on another surface of the substrate, and a through-hole penetrating the at least one cylindrical part along an axis of the at least one cylindrical part such that a diameter of an inner wall of the through-hole gradually decreases from a tip end of the at least one cylindrical part to a fine channel of the plurality of fine channels at a first inclination angle and in communication with the fine channel; and
a resinous covering member bonded to the other surface of the resinous substrate on which the plurality of fine channels is formed;
wherein the microchip is configured to introduce a liquid sample from the tip end of the at least one cylindrical part through the through-hole;
wherein the at least one cylindrical part is formed in an approximate truncated cone shape;
wherein a diameter of an outer wall of the at least one cylindrical part is gradually increased at a second inclination angle from the tip end of the at least one cylindrical part toward the other surface;
wherein the outer wall of the at least one cylindrical part is formed such that the second inclination angle is greater than 0 degree without exceeding 5 degrees; and
wherein an inner wall of the at least one cylindrical part is formed such that an absolute value of the first inclination angle is greater than that of the second inclination angle.
2. The microchip of claim 1 , wherein the first inclination angle of the microchip is 3 degrees or more.
3. A method for producing a microchip comprising:
a molding step for molding a resinous substrate by charging resinous material into a cavity formed by matching a first mold and a second mold, the resinous substrate including:
a plurality of fine channels on a surface of the resinous substrate;
at least one cylindrical part protruding externally from another surface of the substrate; and
a through-hole which penetrates the at least one cylindrical part along an axis of the at least one cylindrical part such that a diameter of an inner wall of the through-hole gradually decreases from a tip end of the at least one cylindrical part to a fine channel of the plurality of fine channels at a first inclination angle, and the through-hole communicates with the fine channel;
a mold releasing step for releasing the resinous substrate subsequent to the molding step from the mold; and
a bonding step for bonding a resinous covering member on the surface provided with the plurality of fine channels of the resinous substrate released from the mold in the mold releasing step;
wherein a portion for forming a fine channel of the plurality of fine channels is formed on one of the first mold and the second mold, and another mold is provided with a concave part for forming the at least one cylindrical part and a pin for forming a taper corresponding to the first inclination angle for forming the through-hole inside the concave part, the pin protruding toward said one of the first mold and the second mold;
wherein during the molding step, a tip end of the pin of the other mold is brought in contact with the fine channel forming portion of said one of the first mold and the second mold, and the resinous material is charged; and
wherein during the mold releasing step, the pin of the other mold is further protruded from a position of contact with the fine channel forming portion toward said one of the first mold and the second mold, whereby the resinous substrate is released from the other mold.
4. The microchip of claim 1 , wherein a wall thickness of the at least one cylindrical part on a tip end side of the at least one cylindrical part to be supplied with a liquid sample is made smaller than a wall thickness on a substrate side of the at least one cylindrical part on which the at least one cylindrical part is formed.