Influenza hemagglutinin and neuraminidase variants
Polypeptides, polynucleotides, methods, compositions, and vaccines comprising (avian pandemic) influenza hemagglutinin and neuraminidase variants are provided.
1. A reassortant influenza virus comprising a genome segment encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 39.
2. The reassortant influenza virus of claim 1 , wherein the genome segment comprises a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 33.
3. The reassortant influenza virus of claim 1 , which is a 6:2 reassortant virus comprising 6 internal genome segments from one or more donor viruses and 2 genome segments encoding surface antigens of an influenza virus.
4. The reassortant influenza virus of claim 3 , wherein the one or more donor viruses are A/Ann Arbor/6/60.
5. The reassortant influenza virus of claim 3 , wherein the one or more donor viruses are selected for one or more phenotypic attributes chosen from attenuated, cold adapted and temperature sensitive.
6. An immunogenic composition comprising an immunologically effective amount of the reassortant influenza virus of claim 1 .
7. A live attenuated influenza vaccine comprising the immunogenic composition of claim 6 .
8. An immunogenic composition comprising an immunologically effective amount of the reassortant influenza virus of claim 3 .
9. A live attenuated influenza vaccine comprising the immunogenic composition of claim 8 .
10. A method for producing reassortant influenza viruses in cell culture, the method comprising:
i) introducing a plurality of vectors comprising polynucleotides corresponding to an influenza virus genome into a population of host cells capable of supporting replication of influenza virus, which plurality of vectors comprises polynucleotides corresponding to at least 6 internal genome segments of a first influenza strain, and at least one genome segment of a second influenza strain, wherein the at least one genome segment of the second influenza strain comprises a polynucleotide comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 39;
ii) culturing the population of host cells; and
iii) recovering a plurality of influenza viruses.
11. The method of claim 10 , wherein the at least one genome segment of the second influenza strain comprises a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 33.
12. The method of claim 10 , wherein the first influenza strain is an attenuated influenza virus strain, a cold-adapted influenza virus strain, or a temperature-sensitive influenza virus strain.
13. The method of claim 10 , wherein the plurality of vectors is eight vectors.
14. The reassortant influenza virus of claim 3 , wherein the one or more donor viruses are other than A/Ann Arbor/6/60.
15. The reassortant influenza virus of claim 14 , wherein the one or more donor viruses are PR8.
16. The reassortant influenza virus of claim 14 , wherein the one or more donor viruses are A/Leningrad/17.
17. The method of claim 10 , wherein the first influenza strain is A/Ann Arbor/6/60.
18. The method of claim 10 , wherein the first influenza strain is other than is A/Ann Arbor/6/60.
19. The method of claim 18 , wherein the first influenza strain is PR8.
20. The method of claim 18 , wherein the first influenza strain is A/Leningrad/17.