Multifunctional encoded particles for high-throughput analysis
Method for making multifunctional particles. The method includes flowing a first monomer stream loaded with a fluorescent entity along a microfluidic channel and flowing a second monomer stream loaded with a probe adjacent to the first monomer stream along the microfluidic channel. The monomer streams are polymerized to synthesize particles having a fluorescent, graphically encoded region and a probe-loaded region.
1. A polymeric particle comprising at least one encoded region and at least one probe-loaded region, the at least one encoded region being a distinct region from the at least one probe-loaded region, wherein the polymeric particle is a PEG hydrogel particle synthesized from a first and second monomer streams; wherein the encoded region is synthesized from the first monomer stream, and the probe-loaded region is synthesized from the second monomer stream loaded with at least one oligonucleotide probe at a concentration of between 50 μM and 150 μM, such that the probe is embedded within the particle and further wherein the particle is greater than about 100 μm up to about 450 μm in at least one dimension.
2. The polymeric particle of claim 1 , wherein the probe includes an inert species to serve as a negative control.
3. The polymeric particle of claim 1 , wherein the encoded region comprises spatially patterned features selected from a plurality of open and closed coding elements.
4. The polymeric particle of claim 3 , wherein the coding elements are arranged in a two-dimensional grid.
5. The polymeric particle of claim 4 , wherein the grid includes 20 coding elements.
6. The polymeric particle of claim 1 , wherein the polymeric particle further comprises at least one orientation indicator.
7. The polymeric particle of claim 3 , wherein the coding elements have non-uniform shapes or sizes.
8. The polymeric particle of claim 1 , comprising more than one probe-loaded region.
9. The polymeric particle of claim 1 , wherein the particle comprises more than one encoded region.
10. The polymeric particle of claim 1 , wherein the PEG hydrogel particle is polymerized from poly(ethylene glycol)diacrylate.
11. The polymeric particle of claim 1 , wherein the at least one encoded region is loaded with one or more fluorescent entities.
12. The polymeric particle of claim 1 , wherein the at least one probe-loaded region comprises multiple probes.
13. The polymeric particle of claim 1 , wherein the probe comprises a linking chemical entity.
14. The polymeric particle of claim 3 , wherein the encoded region comprises graphical encoding, wherein the spatially patterned features allow decoding of the graphically encoded region.
15. The polymeric particle of claim 1 , wherein the encoded region and the probe-loaded region are synthesized from the first and second monomers simultaneously.
16. The polymeric particle of claim 1 , wherein the polymeric particle is porous.
17. The polymeric particle of claim 1 , wherein the polymeric particle is a single-probe particle.
18. The polymeric particle of claim 1 , wherein the probe is incorporated across a sensing region of the polymeric particle.