Myostatin isoform
The present invention provides splice variants of myostatin that promote muscle growth, and include polynucleotides and polypeptide sequences, constructs comprising the sequences and compositions for regulating muscle growth and treating diseases associated with muscle tissue. The splice variants include the consensus sequence X 1 I F L E X 2 X 3 X 4 Q X 5 C S I L X 6 X 7 X 8 X 9 X 10 wherein X 1 is I or L, X 2 is V or L, X 3 is Y, C, G or S, X 4 is I or F, X 5 is F or L, X 6 is G or E, X 7 is E or V, X 8 is A or T, X 9 is A or V and X 10 is absent, F or L. The present invention also provides for the use of the present sequences in identifying animals with altered muscle mass, and for use in selective breeding programs to produce animals with altered muscle mass.
1. An isolated polypeptide comprising an amino acid sequence having the formula:
X 1 I F L E X 2 X 3 X 4 Q X 5 C S I L X 6 X 7 X 8 X 9 X 10 (SEQ ID NO: 134)
wherein X 1 is I or L, X 2 is V or L, X 3 is Y, C, G or S, X 4 is I or F, X 5 is F or L, X 6 is G or E, X 7 is E or V, X 8 is A or T, X 9 is A or V and X 10 is absent, F or L, wherein the polypeptide promotes is capable of promoting myoblast cell growth.
2. An isolated polypeptide that is selected from:
(a) a polypeptide comprising an amino acid sequence of any one of SEQ ID NOS: 48-54, 56-62, 64-66, 68-77, 83-87, 89, 90 and 92-95; and
(b) a polypeptide having at least 90% sequence identity with the polypeptide of (a), wherein the polypeptide promotes myoblast cell growth.
3. A method for regulating muscle growth, comprising administering to a subject in need thereof a composition comprising the polypeptide of claim 1 or 2 .
4. A method for treating muscle cachexia in a subject a disease associated with muscle tissue, comprising administering to the subject in need thereof a composition comprising the polypeptide of claim 1 or 2 .