Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs)
The present invention provides for a recombinant nucleic acid comprising a nucleotide sequence comprising a plurality of constructs, wherein each construct independently comprises a nucleotide sequence of interest flanked by a pair of recombinase recognition sequences. Each pair of recombinase recognition sequences is recognized by a distinct recombinase. Optionally, each construct can, independently, further comprise one or more genes encoding a recombinase capable of recognizing the pair of recombinase recognition sequences of the construct. The recombinase can be an orthogonal (non-cross reacting), site-selective recombinase (OSSR).
1. A recombinant nucleic acid comprising a nucleotide sequence comprising (a) a plurality of constructs comprising a first pair of recombinase recognition sequences which is recognized by a first recombinase, wherein the first pair of recombinase recognition sequences flank a first terminator, and a second pair of recombinase recognition sequences which is recognized by a second recombinase, wherein the second pair of recombinase recognition sequences flank a second terminator, (b) a promoter upstream of the plurality of constructs capable of transcribing the plurality of constructs, wherein each construct independently comprises a nucleotide sequence of interest flanked by a pair of recombinase recognition sequences, wherein each pair of recombinase recognition sequences is recognized by a recombinase that does not cross-react with the recombinase recognition sequences of another construct, and the two recombinase recognition sequences of each pair are oriented in the same direction to each other, and (c) a target gene downstream of the plurality of constructs; wherein the first recombinase and the second recombinase are (i) Escherichia coli Cre and Dre, or (ii) Dre and Saccharomyces cerevisiae FLP.
2. The recombinant nucleic acid of claim 1 wherein each construct independently further comprises one or more genes encoding a recombinase capable of recognizing the pair of recombinase recognition sequences of the construct.
3. A vector comprising the recombinant nucleic acid of claim 1 .
4. An ex vivo host cell comprising the vector of claim 3 .
5. The recombinant nucleic acid of claim 1 , wherein the target gene encodes an open reading frame (ORF), interference RNA, or antisense RNA.
6. The recombinant nucleic acid of claim 5 , wherein the target gene encodes the ORF and the ORF encodes a selective marker, an enzyme, or a polypeptide that cause the death of a host cell.
7. The recombinant nucleic acid of claim 1 , wherein the nucleotide sequences of interest are terminators.
8. The recombinant nucleic acid of claim 1 , wherein each nucleotide of interest comprises (i) an open reading frame (ORF) of a recombinase which recognizes the pair of recombinase recognition sequences flanking the nucleotide of interest, and (ii) a terminator downstream of the ORF.
9. The recombinant nucleic acid of claim 8 , wherein the target gene encodes an open reading frame (ORF) and the ORF encodes a polypeptide that cause the death of a host cell.
10. The recombinant nucleic acid of claim 1 , wherein the first pair of recombinase recognition sequences flank a first gene encoding the first recombinase wherein the first gene is upstream of the first terminator, and the second pair of recombinase recognition sequences flank a second gene encoding the second recombinase wherein the second gene is upstream of the second terminator.