Temperature control device with a flexible temperature control surface
A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.
1. A temperature-control bladder assembly for performing a polymerase chain reaction (PCR), comprising:
a top bracket comprising a first temperature-control bladder;
a bottom bracket comprising a second temperature-control bladder; and
a middle bracket that, when assembled with said top bracket and bottom bracket, defines an interior space,
wherein said first and second bladders form contacts with a reaction chamber placed inside said interior space when inflated with a temperature-control fluid, wherein said reaction chamber is in the form of a PCR tube or a microarray slide.
2. The temperature-control bladder assembly of claim 1 , further comprising a first cushion and a second cushion, wherein said first cushion is placed between said top bracket and said middle bracket and wherein said second cushion is placed between said middle bracket and said bottom bracket.
3. The temperature-control bladder assembly of claim 2 , wherein said first bladder is sealed between said top bracket and said first cushion and wherein said second bladder is sealed between said bottom bracket and said second cushion.
4. The temperature-control bladder assembly of claim 3 , wherein said top bracket is sealed to said first temperature-control bladder with a first o-ring and wherein said bottom bracket is sealed to said second temperature-control bladder with a second o-ring.
5. The temperature-control bladder assembly of claim 1 , wherein each of said top and said bottom brackets comprises an inlet port and an outlet port.
6. The temperature-control bladder assembly of claim 1 , wherein said middle bracket is a U-shaped bracket.
7. The temperature-control bladder assembly of claim 1 , wherein each of said top and said bottom bracket comprises an opening that opens to said interior space defined by said middle bracket, said opening allows a bladder to expand into said interior space and form contact to said reaction chamber.
8. The temperature-control bladder assembly of claim 1 , wherein each of said first and second temperature-control bladder has a shape to accommodate rectangular reaction chamber geometries.
9. A temperature-control bladder assembly for performing a polymerase chain reaction (PCR), comprising:
a top bracket comprising a first temperature-control bladder made with a flexible and heat conductive material;
a bottom bracket comprising a second temperature-control bladder made with a flexible and heat conductive material; and
wherein said first and second bladders form contacts with a reaction chamber placed between said first bladder and said second bladder, wherein said reaction chamber is in the form of a PCR tube or a microarray slide.
10. The temperature-control bladder assembly of claim 9 , further comprising a first cushion and a second cushion, wherein said first cushion is placed between said top bracket and said middle bracket and wherein said second cushion is placed between said middle bracket and said bottom bracket, wherein said first bladder is sealed between said top bracket and said first cushion and wherein said second bladder is sealed between said bottom bracket and said second cushion.
11. The temperature-control bladder assembly of claim 10 , wherein said top bracket is sealed to said first temperature-control bladder with a first o-ring and wherein said bottom bracket is sealed to said second temperature-control bladder with a second o-ring.
12. The temperature-control bladder assembly of claim 9 , wherein each of said top and said bottom brackets comprises an inlet port and an outlet port.
13. The temperature-control bladder assembly of claim 1 , wherein each of said top and said bottom bracket comprises an opening that allows each of said first and said second bladder to expand through said opening and to form contact to said reaction chamber.
14. The temperature-control bladder assembly of claim 9 , wherein each of said first and second temperature-control bladder has a shape to accommodate rectangular reaction chamber geometries.