Reaction plate
A reaction plate includes a welded plastics planar laminate consisting of an aperture plate and a film, the aperture plate having at least one planar surface and a plurality of apertures in the planar surface of the apertured plate and the film being attached to the planar surface of the apertured plate around the or each aperture by welding. The welding is preferably laser or transmission welding. A method of forming such reaction plates using diode laser welding. An apparatus for handling such reaction plates including performing polymerase chain reactions (PCRs) or primer extensions therewith.
1. A method of forming seals around a plurality of wells of a microtitre reaction plate, the wells being defined by a plurality of apertures in a planar surface of the reaction plate through which each well receives a reagent or sample that is subject to one or more reactions while confined and sealed within the reaction plate, each well having a volume of 200 microliters or less, the method comprising the step of attaching a plastics film to the planar surface of the reaction plate by forming seals around the apertures in the planar surface of the reaction plate for sealing the reagent or sample with the wells, using near-infrared diode laser welding to form each well seal, the plate being less than 4 mm thick and having at least 1152 wells per plate.
2. The method of claim 1 , wherein the film covers at least one of the apertures, extending over the planar surface of the apertured reaction plate.
3. The method of claim 1 , wherein at least one of the apertures extends from a first planar surface of the apertured reaction plate to a second, opposed planar surface.
4. The method of claim 3 , wherein a second film is welded to the second surface.
5. The method of claim 4 , wherein both films are transmission welded to the apertured reaction plate.
6. The method of claim 1 , wherein channels or grooves are provided in the apertured reaction plate.
7. The method of claim 1 , wherein the film is optically clear with minimal cross talk.
8. The method of claim 1 , wherein the film has a thickness of between 1 and 500 micrometers.
9. The method of claim 1 , wherein the apertured reaction plate is substantially rigid and opaque, and made of a plastics material.
10. The method of claim 1 , wherein the apertured reaction plate is near infrared absorbent.
11. The method of claim 10 , wherein the apertured reaction plate is black.
12. The method of claim 1 , wherein the apertured reaction plate is non near-infrared absorbent.
13. The method of claim 1 , wherein the film is near-infrared absorbent.
14. The method of claim 1 , wherein, at least one of a reagent and sample are retainable within at least one of the apertures by the film or films.
15. The method of claim 1 , wherein the film is readily pierceable.
16. The method of claim 1 , wherein the film is coated with an affinity reagent.
17. The method of claim 16 , wherein the affinity reagent is streptavidin.
18. The method of claim 1 , wherein the film is coated with a chemical used in a reaction for which the reaction plate is to be used.
19. A method of claim 1 , wherein the apertured reaction plate is substantially rigid and opaque and made of a plastics material.
20. The method of claim 1 , wherein the plate has at least 1440 wells per plate.
21. The method of claim 1 , wherein the plate has at least 1536 wells per plate.