SYSTEMS AND METHODS FOR CHARACTERIZING A BIOLOGICAL CONDITION OR AGENT USING SELECTED GENE EXPRESSION PROFILES
Methods are provided for evaluating a biological condition of a subject using a calibrated profile data set derived from a data set having a plurality of members, each member being a quantitative measure of the amount of a subject's RNA or protein as distinct constituents in a panel of constituents. The biological condition may be a naturally occurring physiological state or may be responsive to treatment of the subject with one or more agents. Calibrated profile data sets may be used as a descriptive record for an agent.
1 .- 86 . (canceled)
87 . A method for determining a profile data set for a subject based on a sample from the subject, the sample providing a source of RNAs, the method comprising:
using amplification for measuring the amount of RNA corresponding to at least two constituents from any one of Tables 1 through 7; and
arriving at a measure of each constituent, wherein the profile data set comprises the measure of each constituent and wherein amplification is performed under measurement conditions (i) that are reproducible such that the coefficient of variation, on repeating the processes of using amplification and arriving at a measure, for the same sample and constituents, is less than approximately 3 percent and (ii) wherein efficiencies of amplification for all constituents differ by less than approximately ten percent.
88 . A method according to claim 1 , wherein efficiencies of amplification for all constituents differ by less than approximately 2 percent.
89 . A method according to claim 1 , wherein efficiencies of amplification for all constituents differ by less than approximately 1 percent.
90 . A method according to according to claim 1 , wherein the sample is of blood from the subject.
91 . A method according to according to claim 1 , wherein the sample is of a blood fraction from the subject.
92 . A method according to according to claim 1 , wherein the sample is of a body fluid from the subject.
93 . A method according to according to claim 1 , wherein the sample is a population of cells from the subject.
94 . A method according to claim 7 , wherein the population of cells is tissue.
95 . A method according to claim 1 , wherein the at least two constituents are selected from Table 1.
96 . A method according to claim 1 , wherein the at least two constituents are selected from Table 2.
97 . A method according to claim 1 , wherein the at least two constituents are selected from Table 3.
98 . A method according to claim 1 , wherein the at least two constituents are selected from Table 4.
99 . A method according to claim 1 , wherein the at least two constituents are selected from Table 5.
100 . A method according to claim 1 , wherein the at least two constituents are selected from Table 6.
101 . A method according to claim 1 , wherein the at least two constituents are selected from Table 7.
102 . A method according to claim 1 , further comprising: storing the data set in a digital storage medium.
103 . A method according to claim 16 , wherein storing the data set includes storing it as a record in a database.