Synthetic dragline spider silk-like proteins
View Patent ↗The present application relates to isolated amino acid sequence comprising multiple repeats of a semi-synthetic spider silk protein domain, or any functional homolog, variant, derivative, fragment or mutant thereof. The amino acid sequence of the invention further comprises an N-terminal region and a C-terminal region. The invention further provides a nucleic acid encoding the amino acid sequence of the invention, an expression vector comprising said nucleic acid, a host cell transformed with said expression vector, a recombinant spider silk protein thus produced and a fiber composed of the recombinant spider silk protein. The invention further encompasses a composition comprising as an active ingredient said amino acid sequence or any said recombinant protein or fiber comprising the same. Lastly, the invention relates to an article comprising at least one fiber composed of said recombinant spider silk protein.
1. An isolated polypeptide comprising n repeats of the amino acid sequence of SEQ ID NO:4, wherein n is an integer above 2.
2. The polypeptide according to claim 1 , wherein n is an integer equal to or below 70.
3. The polypeptide according to claim 2 , wherein n is an integer equal to or between 4 and 32.
4. The polypeptide according to claim 1 , further comprising a C-terminal region having the amino acid sequence of SEQ ID NO:10.
5. The polypeptide according to claim 4 , further comprising an N-terminal region having the amino acid sequence of SEQ ID NO:5.
6. The polypeptide according to claim 5 , further comprising at least one N-terminal tag sequence.
7. An isolated nucleic acid molecule encoding a polypeptide comprising n repeats of the amino acid sequence of SEQ ID NO:4, wherein n is an integer above 2.
8. The nucleic acid molecule according to claim 7 , wherein the amino acid sequence of SEQ ID NO:4 is encoded by the nucleotide sequence of SEQ ID NO:3.
9. The nucleic acid molecule according to claim 7 , wherein the encoded polypeptide further comprises a C-terminal region having the amino acid sequence of SEQ ID NO:10.
10. The nucleic acid molecule according to claim 9 , wherein the amino acid sequence of SEQ ID NO:10 is encoded by the nucleotide sequence of SEQ ID NO:9.
11. The nucleic acid molecule according to claim 9 , wherein the encoded polypeptide further comprises an N-terminal region having the amino acid sequence of SEQ ID NO:5.
12. The nucleic acid molecule according to claim 11 , wherein the amino acid sequence of SEQ ID NO:5 is encoded by the nucleotide sequence of SEQ ID NO:6.
13. An expression vector comprising a nucleic acid molecule encoding a polypeptide comprising n repeats of the amino acid sequence of SEQ ID NO:4, wherein n is an integer above 2, and wherein said polypeptide optionally further comprises at least one of a C-terminal region having the amino acid sequence of SEQ ID NO:10 and an N terminal region having the amino acid sequence of SEQ ID NO:5.
14. An isolated host cell transformed with the expression vector according to claim 13 .
15. An isolated recombinant protein comprising n repeats of the amino acid sequence of SEQ ID NO:4, wherein n is an integer above 2, and wherein said protein optionally further comprises at least one of a C-terminal region having the amino acid sequence of SEQ ID NO:10 and an N-terminal region having the amino acid sequence of SEQ ID NO:5.
16. A fiber comprising the recombinant protein according to claim 15 .
17. A composition comprising:
an isolated polypeptide comprising n repeats of the amino acid sequence of SEQ ID NO:4, wherein n is an integer above 2, wherein said polypeptide optionally further comprises at least one of a C-terminal region having the amino acid sequence of SEQ ID NO:10 and an N-terminal region having the amino acid sequence of SEQ ID NO:5,
a recombinant protein comprising the isolated polypeptide, or
a fiber comprising the isolated polypeptide;
and optionally further comprising a carrier, diluent or excipient.
18. An article comprising at least one fiber, wherein the fiber comprises the recombinant protein according to claim 15 .