System for targeting cells or other materials
An image sensor integrated circuit may contain image sensor pixels. A channel containing a fluid with particles such as cells may be formed on top of the image sensor. Some of the image sensor pixels may form a calibration sensor and some of the image sensor pixels may form an imager. As the fluid and particles flow through the channel at a flow rate, the calibration sensor may measures the flow rate and illumination intensity in the channel. Based on calibration data such as measured flow rate and measured illumination intensity, adjustments may be made to ensure that the imager acquires satisfactory image data. The adjustments may include flow rate adjustments, image acquisition data rate adjustments, and illumination adjustments. A processing unit in the channel may contain a laser or other component to destroy selected cells. A flared region in the channel may be used as a chromatograph.
1. Apparatus, comprising:
an image sensor integrated circuit containing an array of image sensor pixels including calibration sensor pixels and imager sensor pixels;
a fluid channel on the image sensor integrated circuit that is configured to receive fluid;
a calibration sensor formed from the calibration sensor pixels; and
an imager formed from the image sensor pixels.
2. The apparatus defined in claim 1 wherein the calibration sensor is configured to use the calibration sensor pixels to measure a flow rate for the fluid.
3. The apparatus defined in claim 2 wherein the calibration sensor is configured to use the calibration sensor pixels to measure light intensity.
4. The apparatus defined in claim 3 further comprising a light source that illuminates the channel, wherein the calibration sensor is configured to use the calibration sensor pixels to measure light intensity for light emitted by the light source.
5. The apparatus defined in claim 4 further comprising a transparent cover that covers that channel, wherein the light emitted by the light source passes through the transparent cover and wherein the channel has a longitudinal axis and a width perpendicular to the longitudinal axis that is less than 1 mm.
6. The apparatus defined in claim 5 wherein the fluid contains cells and wherein the imager is configured to acquire images of the cells.
7. The apparatus defined in claim 6 further comprising a processing unit in the channel that processes the cells.
8. The apparatus defined in claim 7 wherein the processing unit comprises a laser for destroying the cells after the imager acquires the images of the cells.
9. The apparatus defined in claim 7 wherein the processing unit comprises light sources of different colors.
10. The apparatus defined in claim 7 wherein the processing unit comprises a heater.
11. The apparatus defined in claim 1 wherein the fluid comprises cells and wherein the calibration unit is configured to use the calibration sensor pixels to measure the flow rate of the fluid and the cells and an illumination intensity in the channel, the apparatus further comprising a control circuit that adjusts the imager sensor pixels based at least partly on the measured flow rate and the measured illumination intensity.