Modified host cells with efflux pumps

1. A modified host cell comprising: (a) an efflux pump comprising an amino sequence having at least 95% identity as compared to the amino acid sequence of Azotobacter vinelandii AvOP MexEF, Pseudomonas putida KT2440 TtgABC, Pseudomonas putida KT2440 MexEF-OprN, Pseudomonas aeruginosa PA01 MexAB-OprM, Pseudomonas aeruginosa PA01 MexEF-OprN, Escherichia coli K12 AcrAB, Pseudomonas putida KT2440 NP_745594, Pseudomonas aeruginosa PA01 NP_250708, Marinobacter aqueolei YP_960752, Marinobacter aqueolei YP_957870, or Alcanivorax borkumensis SK2 YP_692684, wherein said efflux pump is heterologous to the modified host cell, and capable of transportation of an organic molecule out of the modified host cell, and (b) a recombinant nucleic acid encoding one or more enzymes for producing the organic molecule; wherein the organic molecule at a sufficiently high concentration inhibits or reduces the growth rate of or is lethal to the modified host cell, the modified host cell is a bacterium and the organic molecule is a C4-C12 alcohol, pinene, geranyl acetate, geraniol, limoene, or faresyl hexanoate.

2. The modified host cell of claim 1 , wherein the efflux pump comprises an amino acid sequence having at least 99% identity as compared to the amino acid sequence of Azotobacter vinelandii AvOP MexEF, Pseudomonas putida KT2440 TtgABC, Pseudomonas putida KT2440 MexEF-OprN, Pseudomonas aeruginosa PA01 MexAB-OprM, Pseudomonas aeruginosa PA01 MexEF-OprN, Escherichia coli K12 AcrAB, Pseudomonas putida KT2440 NP_745594, Pseudomonas aeruginosa PA01 NP_250708, Marinobacter aqueolei YP_960752, Marinobacter aqueolei YP_957870, or Alcanivorax borkumensis SK2 YP_692684.

3. The modified host cell of claim 2 , wherein the efflux pump comprises an amino acid sequence identical to the amino acid sequence of Azotobacter vinelandii AvOP MexEF, Pseudomonas putida KT2440 TtgABC, Pseudomonas putida KT2440 MexEF-OprN, Pseudomonas aeruginosa PA01 MexAB-OprM, Pseudomonas aeruginosa PA01 MexEF-OprN, Escherichia coli K12 AcrAB, Pseudomonas putida KT2440 NP_745594, Pseudomonas aeruginosa PA01 NP_250708, Marinobacter aqueolei YP_960752, Marinobacter aqueolei YP_957870, or Alcanivorax borkumensis SK2 YP_692684.

4. The modified host cell of claim 1 , wherein the efflux pump is able to export the organic molecule from the modified host cell using the proton motive force.

5. The modified host cell of claim 4 , wherein the efflux pump has the amino acid sequence of SEQ ID NO:1, 2, 3, or 4.

6. The modified host cell of claim 4 , wherein the organic molecule is geraniol, and the efflux pump is encoded by Escherichia coli K12 operon encoding AcrAB.

7. The modified host cell of claim 4 , wherein the organic molecule is limonene, and the efflux pump is encoded by Pseudomonas putida KT2440 MexEF-OprN, Pseudomonas aeruginosa PA01 MexAB-OprM, Marinobacter aqueolei operon encoding YP_957870 , Alcanivorax borkumensis SK2 operon encoding YP_692684, or Escherichia coli K12 operon encoding AcrAB.

8. The modified host cell of claim 1 , wherein the efflux pump is comprises an amino sequence identical to the amino acid sequence of Pseudomonas aeruginosa PA01 MexAB-OprM, Pseudomonas putida KT2440 MexEF-OprN, Pseudomonas putida KT2440 TtgABC, or Escherichia coli K12 AcrAB.

9. The modified host cell of claim 1 , wherein the organic molecule is α-pinene or farnesyl hexanoate, and the efflux pump comprises an amino sequence identical to the amino acid sequence of is Azotobacter vinelandii AvOP MexEF, Pseudomonas putida KT2440 TtgABC, Pseudomonas putida KT2440 MexEF-OprN, Pseudomonas aeruginosa PA01 MexAB-OprM, Pseudomonas aeruginosa PA01 MexEF-OprN, Escherichia coli K12 AcrAB, Pseudomonas putida KT2440 NP_745594, Pseudomonas aeruginosa PA01 NP_250708, Marinobacter aqueolei YP_960752, Marinobacter aqueolei YP_957870, or Alcanivorax borkumensis SK2 YP_692684.

10. The modified host cell of claim 1 , wherein the organic molecule is geranyl acetate, geraniol, α-pinene, limoene, or faresyl hexanoate.

11. The modified host cell of claim 1 , wherein the bacterium is a Gram-negative bacterium.

12. The modified host cell of claim 1 , wherein the bacterium is of the genus Planctomyces, Bradyrhizobium, Rhodobacter, Rhizobium, Myxococcus, Klebsiella, Azotobacter, Escherichia, Salmonella, Pseudomonas, Caulobacter, Chlamydia, Acinetobacter, Sinorhizobium, Vibrio , or Zymomonas.

13. The modified host cell claim 1 , wherein the efflux pump comprises an amino acid sequence having at least 95% identity as compared to the amino acid sequence of Escherichia coli K12 AcrAB.

14. The modified host cell claim 1 , wherein the efflux pump comprises an amino acid sequence having at least 95% identity as compared to the amino acid sequence of Pseudomonas putida KT2440 TtgABC.

15. A method for culturing or growing the modified host cell of a present invention, comprising: (a) providing the modified host cell of claim 1 , and (b) culturing or growing the modified host cell such that the efflux pump is expressed, such that the modified host cell has a growth rate or doubling time that is faster compared to the growth rate or doubling time of a cell identical to the modified host cell except that the cell does not express the efflux pump.

16. The method of claim 15 , wherein the culturing or growing step (b) comprises culturing or growing the modified host cell in an environment comprising a sufficiently high concentration of the organic molecule such that the organic molecule inhibits or reduces the growth rate of or is lethal to the host cell.

17. The method of claim 16 , wherein the separating step (c) comprises separating the liquid portion of the solution from the modified host cell in the solution.

18. The method of claim 15 , wherein the method further comprises: (c) separating the organic molecule produced by the modified host cell, and pumped out of the host cell, from the host cell.