Porous electroactive hydrogels and uses thereof
The present invention provides porous electroactive hydrogels, the deformation angle of which is controlled by electroactuation, and methods for preparing and using such hydrogels.
1. A porous electroactive hydrogel, said hydrogel comprising a polyelectrolyte hydrogel monomer comprising an acrylate group, and a polydisperse distribution of pores throughout the hydrogel, wherein said pores have a radius of about 0.5 micrometers to about 10 micrometers, and wherein said hydrogel bends at an angle of greater than about 45 degrees in the presence of an electric field as compared to the bending of the hydrogel in the absence of said electric field, wherein said pores in said hydrogel are prepared by free radical polymerization of the polyelectrolyte monomer in an emulsion.
2. The hydrogel of claim 1 , wherein said electric field comprises about 1-5 volts/cm.
3. The hydrogel of claim 1 , wherein said electric field is applied to the hydrogel for about 2 to about 6 minutes.
4. The hydrogel of claim 1 , wherein said electric field is applied to the hydrogel for about 3 minutes.
5. The hydrogel of claim 1 , wherein said hydrogel comprises an anisotropic pattern.
6. The hydrogel of claim 1 , further comprising an electrode.
7. The hydrogel of claim 1 , further comprising a voltage source.
8. The hydrogel of claim 1 , further comprising a pharmaceutically active compound.
9. The hydrogel of claim 1 , further comprising a chromatophore.
10. The hydrogel of claim 1 , wherein said hydrogel comprises cells.
11. The hydrogel of claim 10 , wherein said hydrogel comprises myocytes.
12. The hydrogel of claim 11 , wherein said myocytes are cardiac myocytes.
13. The hydrogel of claim 11 , wherein said myocytes are skeletal myocytes.
14. A method for preparing the porous electroactive hydrogel of claim 1 , the method comprising
contacting a pre-polymer solution of the polyelectrolyte hydrogel monomer comprising an acrylate group with a water insoluble solution and a surfactant, thereby generating a polymer emulsion, wherein the polymer emulsion comprises about 40-80% by volume of the pre-polymer solution and about 20-60% by volume of the water insoluble solution;
contacting the polymer emulsion with an oxidizing agent, thereby generating a porous electroactive hydrogel and a disperse water insoluble solution; and
removing the disperse water insoluble solution, thereby preparing a porous electroactive hydrogel.
15. The method of claim 14 , further comprising placing a photolithographic mask on top of the polymer emulsion.
16. The method of claim 14 , further comprising placing the polymer emulsion in a mold.
17. The method of claim 16 , wherein the mold comprises a pattern.
18. The method of claim 17 , wherein the pattern comprises grooves of dimensions of about 1 millimeter by about 1 millimeter by about 5 millimeter.
19. The method of claim 14 , further comprising seeding cells on the porous electroactive hydrogel and culturing the cells.
20. The method of claim 14 , further comprising adding a chromatophore to the hydrogel.
21. The method of claim 14 , further comprising adding a protein to the hydrogel.
22. The method of claim 21 , wherein the protein is a pro-apoptotic protein.
23. The method of claim 14 , further comprising seeding said hydrogel with a cell.
24. The method of claim 23 , wherein said cell is selected from the group consisting of a skeletal muscle cell, a smooth muscle cell, and a cardiac muscle cell.
25. A porous electroactive hydrogel prepared according to the method of claim 14 .
26. The hydrogel of claim 1 , wherein said angle is greater than about 90 degrees.
27. The hydrogel of claim 1 , wherein the polyelectrolyte hydrogel monomer comprising an acrylate group is selected from the group consisting of poly(sodium acrylate), poly(ethylene glycol) diacrylate, acrylic acid, polyacrylamide.acrylic acid, hydroxyethyl methacrylate, and derivatives thereof.