Malaria vaccine
The present invention features immunogenic compositions based on pre-fertilization or post-fertilization antigens expressed in the circulating gametocytes in the peripheral blood of infected persons or on the malaria parastes' stages of development in the mosquito midgut including extracellular male and female gametes, fertilized zygote and ookinete. The invention also features methods to prevent the transmission of malaria using the immunogenic compositions of the invention.
1. A method for preparing a codon harmonized Pfs48/45 antigen sequence encoded by a pre-fertilization gene comprising:
Determining the frequency of codon usage of the pre-fertilization gene coding sequence, wherein the Pfs48/45 sequence corresponding to the nucleic acid sequence represented by SEQ ID NO: 7;
Substituting codons in the coding sequence of SEQ ID NO: 7 with codons of similar frequency from a host cell which code for the Pfs48/45 antigen, thereby preparing a codon harmonized Pfs48/45 antigen sequence wherein the codon harmonized Pfs48/45 sequence comprises the nucleotide sequence of SEQ ID NO: 2.
2. An isolated codon harmonized nucleotide sequence that comprises the nucleotide sequence of SEQ ID NO: 2 prepared according to the method of claim 1 .
3. A vector comprising a codon harmonized Pfs48/45 sequence suitable for expression in a cell, wherein the codon harmonized Pfs48/45 sequence comprises the nucleotide sequence of SEQ ID NO: 2.
4. A cell expressing the vector of claim 3 .
5. A kit comprising
an immunogenic composition comprising one or more Plasmodium falciparum or Plasmodium vivax pre-fertilization or post-fertilization antigens, and instructions for use in reducing transmission of Plasmodium falciparum or Plasmodium vivax,
together with one or more of the isolated codon harmonized nucleotide sequence comprising SEQ ID NO 2 and/or the vector of claim 3 .