Dosage forms that facilitate rapid activation of barley protease zymogen
The therapeutic efficacy of zymogen proteins for oral administration is improved by including in the formulation one or more excipients that optimize pH and other reaction conditions for rapid activation of the zymogen shortly after ingestion.
1. A unit dose of a dry pharmaceutical formulation for oral administration of an isolated barley EP-B2 protease zymogen, the formulation comprising:
an effective unit dose of said barley EP-B2 protease zymogen comprising protein content in the range of 10 to 80% of the formulation by weight; and
from 25 to 250 mg of an acid or acidic buffer system, which comprises one or more of
citric acid, maleic acid, malic acid, and fumaric acid and which provides a pH suitable for activation of EP-B2 protease zymogen when the formulation is exposed to gastric fluid;
formulated as capsules, particles or granules in capsules or sachets, or tablets.
2. The formulation of claim 1 , further comprising an antioxidant.
3. The formulation of claim 1 , further comprising a polymer.
4. The formulation of claim 3 , wherein the polymer is an anionic polymer.
5. The formulation of claim 4 , wherein the polymer is selected from the group consisting of dextran sulfate, chondroitin, chondroitin sulfate, heparin, heparin sulfate, cellulose sulfate, xanthan, hyaluronic acid, polyacrylic acid, polyacrylamide, carboxymethylcellulose, polypropanesulfonic acid, polyglutamates, anionic chitosan derivatives, and anionic maleic anhydride copolymers and derivatives.
6. The formulation of claim 5 , wherein the polymer is dextran sulfate at a dose of about 2 μg to about 100 μg.
7. The formulation of claim 1 , wherein the capsule is made of hypromellose.
8. The pharmaceutical formulation of claim 1 , wherein at least 20% of the acid or acidic buffer system is released within 15 minutes of immersing the pharmaceutical formulation in gastric fluid.
9. The pharmaceutical formulation of claim 1 , wherein at least 30% of the barley EP-B2 protease is released in a pulsatile or gradual release profile beginning at least 15 minutes after immersing the pharmaceutical formulation in gastric fluid.
10. A unit dose of a dry pharmaceutical formulation for oral administration of an isolated barley EP-B2 protease zymogen, the formulation comprising:
an effective unit dose of said barley EP-B2 protease zymogen comprising protein content in the range of 10 to 80% of the formulation by weight; and
from 25 to 250 mg of an acid or acidic buffer system, which comprises one or more of citric acid, maleic acid, malic acid, and fumaric acid, and from 1 to 100 mg of sodium metabisulfite;
formulated as capsules, particles or granules in capsules or sachets, or tablets.