Non-transgenic tomato varieties having increased shelf life post-harvest
A series of independent human-induced, non-transgenic mutations found in at least one non-ripening (NOR) gene of tomato; tomato plants having these mutations in at least one of their NOR genes; and a method of creating and identifying similar and/or additional mutations in the NOR gene by screening pooled and/or individual tomato plants. The tomato plants of the present invention exhibit fruit that ripen more slowly, rot more slowly, are firmer, and have a longer shelf life post-harvest as a result of non-transgenic mutations in at least one of their NOR genes.
1. A non-ripening (NOR) gene from tomato comprising a human-induced, non-transgenic G2434A mutation; wherein said human-induced, non-transgenic G2434A mutation comprises a nucleotide change within the NOR gene; and wherein said nucleotide change is identified according to SEQ ID NO: 1.
2. A tomato plant containing a human-induced, non-transgenic mutation within its NOR gene; wherein said human-induced, non-transgenic mutation is G2434A; wherein said human-induced, non-transgenic mutation comprises a nucleotide change within the NOR gene; and wherein said nucleotide change is identified according to SEQ ID NO: 1.
3. The tomato plant of claim 2 , wherein expression of said mutation in the homozygous state results in fruit that are firmer than wild type fruit and turn pink when ripened at standard storage conditions.
4. A Fruit, a seed, a pollen grain, a plant part, or the progeny of the tomato plant of claim 2 ; wherein the fruit, the seed, the pollen grain, the plant part, or the progeny comprises the mutation.
5. A food or a food product comprising the fruit of claim 4 .
6. A NOR protein from tomato comprising a W258* amino acid change; wherein said amino acid change results from a human-induced, non-transgenic mutation in the NOR gene; and wherein said amino acid change is identified according to SEQ ID NO: 2.
7. A tomato fruit comprising a W258* amino acid change in its NOR protein; wherein said amino acid change results from a human-induced, non-transgenic mutation in the NOR gene; and wherein said amino acid change is identified according to SEQ ID NO: 2.
8. The tomato fruit of claim 7 , wherein expression of said amino acid change in its NOR protein in the homozygous state results in fruit that are firmer than wild type fruit and turn pink when ripened at standard storage conditions.
9. A food or a food product comprising the tomato fruit of claim 7 .