Method and device for detection of bioavailable drug concentration in a fluid sample
The invention relates to a method for the controlled delivery of a drug as a function of bioavailable drug concentration, a sensor device for detecting bioavailable drug concentration, and a delivery device that controls delivery of the drug based on the real-time detection of bioavailable drug concentration.
1. An electrochemical, voltammetric sensor device comprising:
at least two or more electrodes and a water-immiscible coating that surrounds the electrodes,
wherein the water-immiscible coating is in contact with the electrodes, wherein the water-immiscible coating comprises about 15 to about 67 wt percent polyvinylchloride (PVC), about 33 to about 85 wt percent 2-nitrophenyl octal ether (o-NPOE), and about 0.001 to about 15 wt percent tetradecylammonium tetrakis(pentoflurorphenyl)borate (TDATPFPB),
wherein the water-immiscible coating partitions a hydrophobic drug from a fluid sample wherein the partitioned drug is oxidized within the water-immiscible coating, wherein the electrodes measure an oxidation/reduction current within the water-immiscible coating, and wherein the measured oxidation/reduction current indicates the concentration of a drug in the fluid sample.
2. The electrochemical, voltammetric sensor device of claim 1 , wherein the electrodes comprise a working electrode, a reference electrode, a counter electrode, or any combination thereof.
3. The electrochemical, voltammetric sensor device of claim 2 , wherein the working electrode comprises glassy carbon, the reference electrode is composed of silver, and the counter electrode comprises platinum.
4. An electrochemical, voltammetric sensor device comprising:
two or more electrodes and a water-immiscible coating that surrounds the electrodes, wherein the water-immiscible coating is in contact with the electrode and comprises about 15 to about 67 wt percent polyvinylchloride (PVC), about 33 to about 85 wt percent 2-nitrophenyl octyl ether (o-NPOE), and about 0.001 to about 15 wt percent tetradecylammonium tetrakis(pentoflurorphenyl)borate (TDATPFPB).
5. The electrochemical, voltammetric sensor device of claim 4 , wherein the two or more electrodes comprise a glassy carbon working electrode, a silver reference electrode, a platinum counter electrode, or any combination thereof.
6. A catheter comprising: a body and a lumen, and an electrochemical, voltammetric sensor device according to claim 1 or 4 in communication with the lumen or externally of the body, wherein the water-immiscible coating is capable of partitioning a bioavailable drug directly from a body fluid.
7. A micro fluidic device comprising a micro fluid channel and an electrochemical, voltammetric sensor device according to claim 1 , or 4 in communication with the microfluid channel.