βGI-IgG intron for enhanced anti-IGF1R expression
View Patent ↗The present invention provides polynucleotides for enhanced expression of a target gene such as an immunoglobulin. Methods of expressing a target gene using the polynucleotides of the invention are also covered.
1. An isolated plasmid vector comprising the nucleotide sequence set forth in SEQ ID NO: 47.
2. An isolated plasmid vector characterized by a plasmid map set forth in FIG. 9 .
3. An isolated host cell comprising the plasmid vector of claim 1 .
4. The host cell of claim 3 wherein the plasmid vector is integrated into the chromosomal DNA of the host cell.
5. The host cell of claim 4 comprising two or more copies of the plasmid vector.
6. The host cell of claim 3 wherein the plasmid vector is not integrated into the chromosomal DNA of the host cell.
7. The host cell of claim 6 comprising two or more copies of the plasmid vector.