Enzymes and methods for producing omega-3 fatty acids
The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.
1. A process for producing oil comprising eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, the process comprising the steps of (i) obtaining seed from a canola plant, the seed comprising eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid which were produced from α-linolenic acid during development of the seed and which are present in an esterified form as part of triglycerides at levels based on an efficiency of conversion of α-linolenic acid to eicosapentaenoic acid, docosapentaenoic acid or docosahexaenoic acid of at least 17.3% and an efficiency of conversion of eicosapentaenoic acid to docosapentaenoic acid of at least 60%, and (ii) extracting oil from the seed so as to thereby produce the oil.
2. The process of claim 1 , wherein arachidonic acid, eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid together comprise at least 15% of the total fatty acid in the oil.
3. The process of claim 2 , wherein the total fatty acid in the oil comprises at least 4% docosahexaenoic acid.
4. The process of claim 3 , wherein at least 6% of the total fatty acid incorporated in triacylglycerol in the oil is docosahexaenoic acid.
5. The process of claim 1 , wherein the step of extracting the oil comprises crushing the seed.
6. The process of claim 5 further comprising a step of degumming, caustic refining, bleaching or deodorizing the extracted oil.
7. The process of claim 1 , wherein docosapentaenoic acid and docosahexaenoic acid are present at levels based on en efficiency of conversion of α-linolenic acid to docosahexaenoic acid of at least 15.4%.
8. The process of claim 1 , wherein docosahexaenoic acid is present at a level based on an efficiency of conversion of α-linolenic acid to docosahexaenoic acid of at least 9.5%.
9. The process of claim 1 , wherein docosahexaenoic acid is present at a level based on an efficiency of conversion of eicosapentaenoic acid to docosahexaenoic acid of at least 45%.