Use of FLT3 ligand for enhancing immune responses in RNA immunization
The invention relates to supplying vaccine RNA to cells. The invention relates in particular to a common use of vaccine RNA and Flt3 ligand for inducing, creating or enhancing an immune response when administered to animals (including humans).
1. An immunogenic preparation, which comprises: (i) isolated mRNA that codes for at least one antigen that induced an immune response in a mammal, and (ii) an immune response intensifying compound that consists of Fms-like tyrosine kinase 3 ligand (Flt3 ligand), wherein said Flt3 ligand is present in an amount effective to enhance the immune response induced by the at least one antigen by at least 10% as compared to an immune response elicited by the isolated mRNA without said immune response intensifying compound.
2. The immunogenic preparation as claimed in claim 1 , wherein the isolated mRNA is synthetic.
3. The immunogenic preparation as claimed in claim 1 , which further comprises at least one RNA-stabilizing factor.
4. A pharmaceutical composition that comprises an immunogenic preparation as claimed in claim 1 in a pharmaceutically compatible diluent or a pharmaceutically compatible vehicle, or both.
5. The pharmaceutical composition as claimed in claim 4 in the form of a formulation as a vaccine.
6. The pharmaceutical composition as claimed in claim 4 in the form of a formulation for intranodal administration.