Method for treating prostatitis utilizing modified pore-forming protein proaerolysin
The present disclosure includes methods and compositions for treating any condition involving prostatitis and similar diseases and/or conditions. These methods and compositions involve the use of targeted modified pore-forming proteins, including variant proaerolysin proteins.
1. A method of treating prostatitis in a subject, comprising administering to said subject a therapeutically effective amount of PRX302,
wherein the PRX302 comprises:
an amino acid sequence having greater than 98% sequence identity to the amino acid sequence shown in SEQ ID NO:4 and which maintains the ability to selectively target and kill normal prostate cells, and
an affinity tag.
2. The method of claim 1 , wherein the affinity tag is a polyhistidine tag.
3. The method of claim 2 , wherein the polyhistidine tag includes six histidine residues.
4. The method of claim 1 , wherein the PRX302 comprising an amino acid sequence having greater than 98% sequence identity to the amino acid sequence shown in SEQ ID NO:4, which maintains the ability to selectively target and kill normal prostate cells, and an affinity tag is administered intravenously, intraprostatically, intramuscularly, subcutaneously, or orally.
5. The method of claim 4 , wherein the PRX302 comprising an amino acid sequence having greater than 98% sequence identity to the amino acid sequence shown in SEQ ID NO:4, which maintains the ability to selectively target and kill normal prostate cells, and an affinity tag is administered intravenously at 1 to 10 mg per 70 kg body weight.
6. The method of claim 4 , wherein the PRX302 comprising an amino acid sequence having greater than 98% sequence identity to the amino acid sequence shown in SEQ ID NO:4, which maintains the ability to selectively target and kill normal prostate cells, and an affinity tag is administered intravenously at about3 mg per 70 kg body weight.
7. The method of claim 4 , wherein the PRX302 comprising an amino acid sequence having greater than 98% sequence identity to the amino acid sequence shown in SEQ ID NO:4, which maintains the ability to selectively target and kill normal prostate cells, and an affinity tag is administered intraprostatically at 1 to 100 mg per 70 kg body weight.
8. The method of claim 7 , wherein the PRX302 comprising an amino acid sequence having greater than 98% sequence identity to the amino acid sequence shown in SEQ ID NO:4, which maintains the ability to selectively target and kill normal prostate cells, and an affinity tag is administered intraprostatically at 25 to 30 mg per 70 kg body weight.
9. The method of claim 7 , wherein the PRX302 comprising an amino acid sequence having greater than 98% sequence identity to the amino acid sequence shown in SEQ ID NO:4, which maintains the ability to selectively target and kill normal prostate cells, and an affinity tag is administered intraprostatically at a volume equivalent to 20% of the prostate volume, at a fixed concentration of 3 μg/mL with the total dose being about 30 μg per gram prostate.
10. The method of claim 1 , wherein administration results in a reduction in prostate volume.
11. The method of claim 10 , wherein administration results in at least a 10% reduction in prostate volume.
12. The method of claim 10 , wherein administration results in at least a 50% reduction in prostate volume.
13. The method of claim 1 , further comprising selecting a subject with at least one or more symptoms associated with prostatitis.
14. The method of claim 1 , wherein the prostatitis is chronic prostatitis.
15. The method of claim 1 , further comprising administering an additional therapeutic agent for treating prostatitis.
16. The method of claim 15 , wherein the additional therapeutic agent comprises an α-1-adrenoreceptor antagonist, an antibiotic, an anti-inflammatory agent, a 5α reductase inhibitor, a phytotherapy, or any combination thereof.
17. The method of claim 1 , further comprising selecting a subject with at least one or more signs associated with prostatitis.
18. The method of claim 17 , wherein the one or more signs associated with prostatitis comprises a prostate volume of 30 to 100 mL, prostate specific antigen blood levels of 4-10 ng/mL, or a combination thereof.
19. The method of claim 3 , wherein the six histidine residues tag is at the C-terminal end of PRX302 (SEQ ID NO:4).
20. A method of treating prostatitis in a subject, comprising administering to said subject a therapeutically effective amount of PRX302,
wherein the PRX302 comprises:
an amino acid sequence having greater than 98% sequence identity to the amino acid sequence shown in SEQ ID NO:4 and which maintains the ability to selectively target and kill normal prostate cells, and
a C-terminal polyhistidine tag that includes six histidine residues.