Polypeptides with xylanase activity
View Patent ↗Polypeptides with xylanase activity modified to increase bran solubilization and/or xylanase activity. The modification comprises at least an amino acid modification i position 110 and may have further modifications of one or more amino acids in position 11, 12, 13, 34, 54, 77, 81, 99, 104, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166 or 175 wherein the positions are determined as the position corresponding the position of Bacillus subtilis xylanase (SEQ ID NO 1) The polypeptides have at least 88% identity with SEQ ID NO 1 or 75% identity to a sequence selected from SEQ ID NO 2-22.
1. A polypeptide having xylanase activity and comprising an amino acid sequence, said amino acid sequence having at least 88% identity with SEQ ID No. 1, and which polypeptide has an amino acid substitution in position 110, with any one different amino acid residue selected from the group consisting of: asparagine (N), glutamic acid (E), tryptophan (W), alanine (A) and cysteine (C), and one or more amino acid substitutions selected from the group consisting of: 11F, 12F, 122D, 113A, 13Y, 54Q, 54W, 113D, 141Q, 175L, 122F, 34K, 99Y, 104W, 154R, 159D, 175K, 81I, 166F, 162E, 162D, 164F, 114D, 114Y, 114F, 118V, 175K, 77L, 77M, 77S, 77V, and 77Y, the position(s) being determined as the corresponding position(s) of B. subtilis amino acid sequence shown as SEQ ID No. 1.
2. A polypeptide according to claim 1 having xylanase activity and comprising an amino acid sequence, said amino acid sequence having at least 90% identity with SEQ ID No. 1 and which polypeptide has an amino acid modification in position 110, wherein said position 110 is determined as the position corresponding to position 110 of B. subtilis xylanase sequence shown as SEQ ID No. 1 by alignment.
3. The polypeptide according to claim 2 , wherein said polypeptide has at least 95% identity with SEQ ID No. 1.
4. The polypeptide according to claim 1 , having a β-jelly roll fold.
5. The polypeptide according to claim 1 , wherein the amino acid modification in position 110 is a substitution to alanine.
6. The polypeptide according to claim 1 , having a total number of amino acids of less than 250.
7. A composition comprising the polypeptide according to claim 1 admixed with a non-toxic component.