Immunoanalytical method and system using mass spectrometry technology
View Patent ↗An immunoanalytical system in which, after a sample such as a patient's serum is subjected to a pretreatment by an immunological pretreatment device, the sample is subjected to light detection by an immunological photometric detection system. Subsequently, the mass spectrometric pretreatment device performs a pretreatment, and the mass spectrometric detection system performs mass spectrometry. The mass spectrometric detection system performs mass spectrometry on components contained in a supernatant. A signal intensity and peak area for each of components are calculated from an obtained chromatograph. A quantitative value measured based on the immunoanalytical method is calculated for each of the components on the basis of the relative ratios of the components.
1. An immunoanalytical method comprising the steps of:
capturing an object ( 101 , 501 , 1032 ) to be measured, the object being contained in a sample solution, by using an antibody during a pretreatment process based on the immunoanalytical method;
quantifying the captured object ( 101 , 501 , 1032 ) to be measured;
collecting the object ( 101 , 501 , 1032 ) to be measured from a waste liquid resulting after the captured object to be measured has been subjected to the quantification; and
performing mass spectrometry on the collected object to be measured in conformity with a mass spectrometry method and measuring components of the object measured based on the immunoanalytical method.
2. The immunoanalytical method according to claim 1 ,
wherein, during a pretreatment process based on the immunoanalytical method, the object that is to be measured and is contained in the sample solution is captured by using a primary antibody ( 204 ), a secondary antibody ( 206 ), and a magnetic particle ( 201 ), and the captured object to be measured is irradiated with light for quantification.
3. The immunoanalytical method according to claim 2 ,
Wherein, the quantified object to be measured is collected by using the primary antibody ( 204 ), the secondary antibody ( 206 ), and the magnetic particle ( 201 ).
4. The immunoanalytical method according to claim 2 ,
wherein proportions of the components of the object measured based on the immunoanalytical method are calculated by the mass spectrometry method, and a quantitative value for each of the components is corrected by multiplying the quantitative value calculated by the immunoanalytical method by the proportion of the component.
5. The immunoanalytical method according to claim 1 , further comprising the steps of:
capturing an antigen by an antigen-antibody reaction from a waste liquid resulting after the captured object to be measured has been subjected to the quantification; and
collecting the captured antigen after the antigen is liberated,
wherein the mass spectrometry is performed on the collected object to be measured based on the mass spectrometry method.