Nanofabricated membrane using polymerized proteoliposomes
The present invention generally related to a nanofabricated membrane including polymerized proteoliposomes. The nanofabricated membrane is a bio-nano fused selective membrane using protein-incorporated uv-crosslinkable liposomes with a chemical reactive biocompatible interstitial matrix. In the present invention, internally UV-crosslinked protein-incorporated proteolipsomes are used because the proteoliposomes made by natural lipids have a short life time and a weak resistance to the circumstantial stresses such as a high and low temperature, pressure, ionic strength etc. Furthermore, the proteo-vesicles made by amphiphilic block copolymers provide less consistency in accomplishing proper functionality batch to batch because of the inevitable polydiversity of the polymer.
1. A membrane including liposomes supported by a base porous membrane, said liposomes having proteins incorporated into their walls wherein said liposomes are internally UV-crosslinked and wherein there is external cross-linking between liposomes or between the liposomes and an interstitial matrix or a support, said external cross-linking being via the hydrophilic region of the liposomes.
2. The membrane of claim 1 , wherein the liposomes comprise lipids having two hydrophobic tails and the internal UV-crosslinks are included in one or both of the hydrophobic tails.
3. The membrane of claim 1 , wherein the base membrane comprises a mixed cellulose ester, cellulose, and/or polyamide.
4. The membrane according to claim 1 , wherein proteins of the Aquaporin family of proteins are incorporated into the liposome, the membrane being a water filtration membrane.
5. A membrane according to claim 1 , in which the base membrane comprises a thin polyamide layer.
6. The membrane of claim 1 , in which there is external cross-linking between liposomes.
7. The membrane of claim 5 , wherein the liposomes are linked to the polyamide thin layer.