Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabinogalactan-proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.
1. An emulsion system, comprising:
(A) a plant-expressed fusion protein in aqueous solution, said protein having emulsifying activity and comprising (i) a first glycomodule of Xaa-Hyp-Xaa-Hyp (SEQ ID NO:9) and (ii) a second glycomodule selected from Xaa-Hyp-Hyp n (SEQ ID NO:209) and Xaa-Pro-Hyp n (SEQ ID NO: 210);
wherein n is selected from 2 to 100 and Xaa is selected from Ser, Thr, and Ala;
wherein the first glycomodule is hydroxyproline O-glycosylated with at least one arabinogalactan polysaccharide; and
(B) an oil.
2. The emulsion system according to claim 1 , wherein the second glycomodule is hydroxyproline O-arabinosylated with at least one arabinoside.
3. The emulsion system according to claim 1 , wherein the fusion protein has emulsion stabilizing activity.
4. The emulsion system according to claim 1 , wherein the fusion protein comprises at least a portion of a non-gum arabic glycoprotein.
5. The emulsion system according to claim 1 , wherein said fusion protein is suitable for use in food.
6. The emulsion system according to claim 1 , wherein said system is a water-in-oil emulsion.
7. The emulsion system according to claim 1 , wherein said system is an oil-in-water emulsion.
8. A method of preparing an emulsion, comprising:
(A) providing a plant-expressed fusion protein in aqueous solution, said protein having emulsifying activity and comprising (i) a first glycomodule of Xaa-Hyp-Xaa-Hyp (SEQ ID NO:9) and (ii) a second glycomodule selected from Xaa-Hyp-Hyp n (SEQ ID NO:209) and Xaa-Pro-Hyp n (SEQ ID NO: 210);
wherein n is selected from 2 to 100 and Xaa is selected from Ser, Thr, and Ala;
wherein the first glycomodule is hydroxyproline O-glycosylated with at least one arabinogalactan polysaccharide; and
(B) combining the provided aqueous solution with an oil; and
(C) forming an emulsion.
9. The method according to claim 8 , wherein the second glycomodule is hydroxyproline O-arabinosylated with at least one arabinoside.
10. The method according to claim 8 , wherein the aqueous solution comprises a buffer.
11. The method according to claim 8 , wherein the fusion protein comprises at least a portion of a non-gum arabic glycoprotein.
12. The method according to claim 8 , wherein the fusion protein has emulsion stabilizing activity.
13. The method according to claim 8 , wherein said formed emulsion is a water-in-oil emulsion.
14. The method according to claim 8 , wherein said formed emulsion is an oil-in-water emulsion.