Modular assay plates, reader systems and methods for test measurements
Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.
1. A method for carrying out an electrode induced luminescence assay comprising:
(a) inserting one or more assay modules into a plate frame of a multi-well plate, said plate frame defining two or more apertures for receiving and holding one or more assay modules, each assay module having
(i) an array of wells with integrated electrodes,
(ii) a module lip,
(iii) a snap fit element extending from said module, said snap fit element positioned below said lip, wherein said lip and said snap fit element are configured to engage said plate frame,
(iv) a module top having a plurality of through-holes that define the walls of said array of wells, and
(v) a module bottom defining the bottom of said array of wells,
and said plate frame having a frame lip that extends into said apertures, thereby engaging said module lip and said snap fit element within said plate frame
(b) introducing one or more samples into one or more wells of said multi-well plate; and
(c) applying an electrical potential to said electrodes and measuring luminescence produced in said wells.
2. The method of claim 1 , further comprising inserting said one or more modules in said plate frame.
3. The method of claim 1 , wherein at least one of said wells comprises a binding reagent for an analyte of interest.
4. The method of claim 3 , wherein said binding reagent is labeled with an electrochemiluminescent label.
5. The method of claim 3 , wherein said binding reagent is immobilized on working electrodes within said wells.
6. The method of claim 1 , wherein one sample is distributed into wells on a plurality of said assay modules and at least two of said plurality of assay modules being configured for carrying out different panels of assays.
7. The method of claim 1 , wherein a plurality of samples are distributed into a plurality of said assay modules, including at least a first sample that is distributed into wells of a first of said assay modules and a second sample that is distributed into wells of a second of said assay modules, said first and second assay modules being configured for carrying out the same panel of assays.
8. The method of claim 1 , wherein said one or more samples are introduced into a plurality of said assay modules and said step of applying electrical potential and measuring luminescence is conducted sequentially on each of said assay modules.
9. A method for carrying out an electrode induced luminescence assay comprising:
(a) introducing one or more samples into one or more wells of one or more assay modules, the assay modules having an array of wells with integrated electrodes, the one or more assay modules comprising: a module lip and a snap fit element extending from said module, said snap fit element positioned below said lip, wherein said lip and said snap fit element are configured to engage a plate frame of a multi-well plate; a module top having a plurality of through-holes that define the walls of said array of wells; and a module bottom defining the bottom of said array of wells;
(b) inserting said assay modules into a plate frame that defines one or more apertures for receiving and holding said multi-well assay modules and thereby engaging said module lip and said snap fit element within said plate frame; and
(c) applying an electrical potential to said electrodes and measuring luminescence produced in said wells.
10. The method of claim 9 , further comprising inserting said one or more modules in said plate frame.
11. The method of claim 9 , wherein at least one of said wells comprises a binding reagent for an analyte of interest.
12. The method of claim 9 , wherein one sample is distributed into wells on a plurality of said assay modules and at least two of said plurality of assay modules being configured for carrying out different panels of assays.
13. The method of claim 9 , wherein a plurality of samples are distributed into a plurality of said assay modules, including at least a first sample that is distributed into wells of a first of said assay modules and a second sample that is distributed into wells of a second of said assay modules, said first and second assay modules being configured for carrying out the same panel of assays.