Gemcitabine immunoassay
The present invention comprises novel conjugates and immunogens derived from gemcitabine and unique antibodies generated by using gemcitabine linked immunogens, which conjugates immunogens and antibodies, are useful in immunoassays for the quantification and monitoring of gemcitabine in biological fluids.
1. An antibody which binds selectively to gemcitabine and does not have any substantially cross reactivity to 2′, 2′-difluoro-2′-deoxyuridine and tetrahydrouridine.
2. The antibody of claim 1 wherein said antibody has a cross-reactivity with regard to 2′, 2′-difluoro-2′-deoxyuridine and tetrahydrouridine of less than 20%, based upon said antibody's reactivity with gemcitabine.
3. The antibody of claim 2 wherein said cross reactivity is less than 10%.
4. The antibody of claim 1 , wherein said antibody is derived from mice, sheep, rabbits or rats.
5. The antibody of claim 4 , wherein said antibody is a monoclonal antibody.
6. The antibody of claim 1 , wherein said antibody is derived from an immunogenic carrier having a reactive amino or thiol group polymer conjugated to a compound selected from the group consisting of compounds of the formula:
wherein B is —CH 2 — or
Y is an organic spacing group;
X is a functional group capable of binding to said carrier through said amino or thiol group; and
p is an integer from 0 to 1 and a salt of the compound of formula IV.
7. The antibody of claim 6 , wherein the carrier contains a thiol group and X in the compound which is conjugated to the immunogenic polymer is a functional group capable of reacting with said thiol.
8. The antibody of claim 7 , wherein X in said compound is
9. The antibody of claim 8 , wherein Y in said compound is lower alkylene.
10. The antibody of claim 9 , wherein the immunogenic carrier contains as the functional group:
Wherein v is an integer from 1 to 6.
11. The antibody of claim 10 , wherein said antibody is derived from mice, sheep, rabbits or rats.