Antitoxin and vaccine platform based on nodavirus VLPS
Antitoxin and vaccine compositions based on nodavirus VLPs are provided. Anthrax antitoxin and vaccine compositions are provided. Methods of treating toxins with VLP-based antitoxins are provided. Methods of raising an immune response with immunogen decorated VLPs are provided.
1. An immunogenic composition comprising a nodavirus-derived virus like particle (VLP) that comprises a heterologous immunogen binding domain, said heterologous binding domain comprising an anthrax binding sequence of an extracellular von Willebrand factor A (VWA) domain, which binding domain is bound to a first heterologous immunogen, wherein the heterologous immunogen comprises a first domain and a second domain, wherein the first domain comprises an anthrax protective antigen and the second domain comprises a botulinum antigen.
2. The immunogenic composition of claim 1 , wherein the VLP is derived from Flock House Virus (FHV).
3. The immunogenic composition of claim 1 , wherein the VLP comprises 180 binding domains coupled to up to a maximum of about 120 heterologous immunogens.
4. The immunogenic composition of claim 1 , wherein the binding domain comprises a VWA domain of capillary morphogenesis protein 2 (ANTRX2).
5. The immunogenic composition of claim 4 , wherein the first and second domains are recombinantly expressed as a fusion protein, and the first domain is bound by the VWA domain of ANTRX2.
6. The immunogenic composition of claim 1 , wherein the VLP comprises a plurality of heterologous immunogen binding domains on the surface of the VLP, wherein a first and second heterologous immunogen are each bound, respectively, to one of the heterologous immunogen binding domains, wherein said first and second heterologous immunogen each comprising a first domain and a second domain, wherein the first domain comprises an anthrax protective antigen and the second domain comprises an antigen selected from ricin toxin, botulinum toxin and other immunogens, wherein the second domain of the first and second heterologous immunogens are different.
7. The immunogenic composition of claim 6 , wherein the second domain of the first heterologous immunogen comprises a domain derived from botulinum A toxin and the second domain of the second heterologous immunogen comprises a domain derived from ricin toxin.
8. An immunogenic composition comprising a nodavirus-derived virus like particle (VLP) derived from Flock House Virus, said VLP comprising a heterologous immunogen binding domain, said binding domain being bound to a first heterologous immunogen, said heterologous immunogen comprising a first anthrax protective antigen domain coupled to a second heterologous domain comprising a botulinum toxin.
9. An immunogenic composition comprising a first and second nodavirus-derived virus like particle (VLP) wherein the first VLP is different from the second VLP, wherein the first and second VLPs each comprise a VWA domain of capillary morphogenesis protein 2 (ANTRX2) bound to a respective first and second heterologous immunogen, further wherein the first and second heterologous immunogen each comprise a fusion protein, said fusion protein comprising a first domain derived from anthrax protective antigen and a second domain selected from a ricin toxin antigen, botulinum toxin antigen, or other immunogen of interest, wherein the second domain of the first and second heterologous immunogens are different.
10. The immunogenic composition of claim 9 , wherein the first and second VLPs are each is derived from Flock House Virus (FHV).
11. The immunogenic composition of claim 8 , wherein the heterologous binding domain comprises a VWA domain of capillary morphogenesis protein 2.