Surgical microscope system and method
A microscopy system and method allow observing a fluorescent substance accumulated in a tissue. The tissue can be observed at a same time both with visible light and with fluorescent light. It is possible to observe a series of previously recorded fluorescent light images in superposition with the visible light images. An end of the series of images may be automatically determined. A thermal protective filter may be inserted into a beam path of an illuminating system at such automatically determined end of the series. Further, the fluorescent light image may be analyzed for identifying a coherent fluorescent portion thereof. A representation of a periphery line of the coherent portion may be generated, and depths profile data may be obtained only from the coherent portion. An illuminating light beam for exciting the fluorescence may be modulated for improving a contrast of fluorescent images.
1. A method of operating a surgical microscope, wherein the surgical microscope comprises:
a camera having a light detecting component;
microscopy optics having a first beam path for optically imaging an object region onto the light detecting component of the camera for generating image data representing intensities of images of the object region; and
an illumination system configured to direct at least one illuminating light beam onto the object region;
wherein the illumination light beam includes visible light from a broad wavelength range and excitation light of a fluorescence of indocyanine green of about 800 nm;
wherein the illumination system comprises a first filter which is positionable at the first filter's first position in which the first filter is disposed in the illumination light beam and the first filter's second position in which the first filter is not disposed in the illumination light beam;
wherein the first filter is configured to eliminate light with wavelengths greater than a predetermined wavelength from the illuminating light beam and to allow the visible light of wavelengths up to the predetermined wavelength to traverse the first filter, wherein the predetermined wavelength is in a range of one of 690 nm to 780 nm;
wherein the illumination system comprises a second filter which is positionable at the second filter's fall first position in which the first filter is disposed in the illumination light beam;
wherein the second filter is configured to eliminate light with wavelengths greater than 800 nm from the illuminating light beam and to allow light of wavelengths up to 800 nm to traverse the second filter; and
wherein the method comprises:
providing a combined fluorescence and visible light observation mode of the surgical microscope by positioning the first filter in its second position and the second filter in its first position; then
recording at least one fluorescence image of the object region using the camera; and then
terminating the combined fluorescence and visible light observation mode and providing a pure visible light observation mode by moving the first filter from its second position to its first position.
2. The method according to claim 1 , wherein the terminating of the combined fluorescence and visible light observation mode and providing the pure visible light observation mode comprises moving the second filter from its first position to the second filter's second position.
3. The method according to claim 2 , wherein the terminating of the combined fluorescence and visible light observation mode and providing the pure visible light observation mode comprises moving the first and second filters between first and second configurations, wherein the first filter is in its first position and the second filter is in its second position in the first configuration and wherein the first filter in its second position and the second filter is in its first position in the second configuration.
4. The method according to claim 1 , further comprising a control device configured to analyze the image data and to control the movement of the first filter from its second position to its first position based on an analysis of the image data.
5. The method according to claim 4 , wherein the control device is configured to analyze the intensities of the images represented by the image data and to control the movement of the first filter from its second position to its first position based on an analysis of the intensities of the images.
6. The method according to claim 4 , wherein the control device is configured to analyze changes of the intensities of the images represented by the image data and to control the movement of the first filter from its second position to its first position based on an analysis of the changes of the intensities of the images.
7. The method according to claim 1 , wherein the image data represent images of the object region with light including wavelengths of a wavelength range comprising a fluorescent emission wavelength of the indocyanine green accumulated in the object region.
8. The method according to claim 7 , wherein the fluorescent emission wavelength of the indocyanine green is greater than the predetermined wavelength.
9. The method according to claim 1 , wherein the illumination system includes a light source emitting the visible light, the excitation light and thermal radiation.
10. The method according to claim 9 , wherein the light source is one of a xenon lamp and a halogen lamp.
11. The method according to claim 1 , wherein the microscopy optics has at least one second beam path including an ocular for optically imaging object region to a user's via the ocular.
12. A surgical microscope comprising:
a camera having a light detecting component;
microscopy optics having a first beam path for optically imaging an object region onto the light detecting component of the camera for generating image data representing intensities of images of the object region; and
an illumination system configured to direct at least one illuminating light beam onto the object region;
wherein the illumination light beam includes visible light from a broad wavelength range and excitation light of a fluorescence of indocyanine green of about 800 nm;
wherein the illumination system comprises a first filter which is positionable at the first filter's first position in which the first filter is disposed in the illumination light beam and the first filter's second position in which the first filter is not disposed in the illumination light beam;
wherein the first filter is configured to eliminate light with wavelengths greater than a predetermined wavelength from the illuminating light beam and to allow the visible light of wavelengths up to the predetermined wavelength to traverse the first filter, wherein the predetermined wavelength is in a range from 690 nm to 780 nm;
wherein the illumination system comprises a second filter which is positionable at the second filter's first position in which the first filter is disposed in the illumination light beam;
wherein the second filter is configured to eliminate light with wavelengths greater than 800 nm from the illuminating light beam and to allow light of wavelengths up to 800 nm to traverse the second filter; and
further comprising a combined fluorescence and visible light observation mode of the surgical microscope by positioning the first filter in its second position and the second filter in its first position;
wherein at least one fluorescence image of the object region using the camera is recorded; and
wherein the combined fluorescence and visible light observation mode is terminated and a pure visible light observation mode is provided by moving the first filter from its second position to its first position.
13. The surgical microscope according to claim 12 , wherein the combined fluorescence and visible light observation mode is terminated and the pure visible light observation mode is provided by moving the second filter from its first position to the second filter's second position.
14. The surgical microscope according to claim 13 , wherein the combined fluorescence and visible light observation mode is terminated and the pure visible light observation mode is provided by moving the first and second filters between first and second configurations, wherein the first filter is in its first position and the second filter is in its second position in the first configuration and wherein the first filter in its second position and the second filter is in its first position in the second configuration.
15. The surgical microscope according to claim 12 , further comprising a control device configured to analyze the image data and to control the movement of the first filter from its second position to its first position based on an analysis of the image data.
16. The surgical microscope according to claim 15 , wherein the control device is configured to analyze the intensities of the images represented by the image data and to control the movement of the first filter from its second position to its first position based on an analysis of the intensities of the images.
17. The surgical microscope according to claim 15 , wherein the control device is configured to analyze changes of the intensities of the images represented by the image data and to control the movement of the first filter from its second position to its first position based on an analysis of the changes of the intensities of the images.
18. The surgical microscope according to claim 12 , wherein the image data represent images of the object region with light including wavelengths of a wavelength range comprising a fluorescent emission wavelength of the indocyanine green accumulated in the object region.
19. The surgical microscope according to claim 18 , wherein the fluorescent emission wavelength of the indocyanine green is greater than the predetermined wavelength.
20. The surgical microscope according to claim 12 , wherein the illumination system includes a light source emitting the visible light, the excitation light and thermal radiation.
21. The surgical microscope according to claim 20 , wherein the light source is one of a xenon lamp and a halogen lamp.
22. The surgical microscope according to claim 12 , wherein the microscopy optics has at least one second beam path including an ocular for optically imaging object region to a user's via the ocular.