Microfluidic chemostat
A chemostat is described that includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat is described. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.
1. A method of preventing biofilm formation in a growth chamber of a chemostat, the method comprising:
isolating a portion of the growth chamber;
adding a lysis agent to the isolated portion of the growth chamber;
flushing the isolated portion with growth medium for a period of time; and
reuniting the isolated portion of the growth chamber with the rest of the growth chamber.
2. The method of claim 1 , wherein isolating a portion of the growth chamber further comprises closing actuable valves that fluidly isolate the portion from the rest of the growth chamber.
3. The method of claim 2 , wherein adding a lysis agent to the isolated portion of the growth chamber further comprises opening an actuable supply valve to supply the lysis agent to the isolated portion of the growth chamber.
4. The method of claim 1 , further comprising removing the lysis agent from the isolated portion of the growth chamber prior to flushing the isolated portion with fresh sterile growth medium.
5. The method of claim 1 , further comprising filling the isolated portion with growth medium prior to reuniting the isolated portion with the rest of the growth chamber.