CIP2A as a biomarker in detection of cervical cancer
The present invention provides methods and compositions for detecting cervical cancer in a human. The present invention provides CIP2A as a biomarker detectable in a biological sample from cervix of an individual, an increased expression of same is an indication of cervical cancer in the individual. CIP2A mRNA or protein can both serve as reliable biomarkers. In one embodiment, the present invention provides a combined use of CIP2A with at least one additional biomarker selected from the group consisting of Ki67, TOP2A, MCM2, MCM5, p14 ARF and p16 INK4a , to better serve as a detection means for cervical cancer. The present invention provides a reliable and accurate assay methods and compositions which may be used for detecting cervical cancer in an individual with a high sensitivity and specificity. The present assay provides a novel use of CIP2A as a tool for early detection of cervical cancer in humans.
1. A method of detecting cervical cancer in a human, comprising the steps of:
(a) obtaining a cervical sample from a human suspected of suffering from cervical cancer;
(b) isolating nucleic acid from said cervical sample;
(c) performing a molecular assay to detect an expression level of cancerous inhibitor of protein phosphatase 2A (CIP2A) in said cervical sample, said molecular assay is selected from the group consisting of quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis;
(d) detecting cervical cancer in said human based on said molecular assay that has a sensitivity of at least 80% and a specificity of >90%,
wherein an increase in expression level of said CIP2A as evidenced by said molecular assay is indicative of cervical cancer in said human.
2. The method of claim 1 , wherein said cervical sample is obtained with a swab.
3. The method of claim 1 , wherein said cervical sample is a tissue.
4. The method of claim 1 , wherein said CIP2A is a mRNA or a protein.
5. The method of claim 4 , wherein said CIP2A is a mRNA.
6. The method of claim 4 , wherein said CIP2A is a protein.
7. The method of claim 4 , wherein said molecular assay is qRT-PCR.
8. The method of claim 4 , wherein said molecular assay is Western blot analysis.
9. The method of claim 1 , further comprising the step of:
(e) detecting an expression level of a cervical cancer biomarker selected from the group consisting of Ki67, topoisomerase 2-alpha (TOP2A), minichromosome maintenance complex component 2(MCM2), minichromosome maintenance complex component 5(MCM5), p14 ARF and p16 INK4a in said cervical sample,
wherein an increase in expression level of CIP2A in combination with an increase in expression level of said cervical cancer biomarker is indicative of cervical cancer in said human.
10. The method of claim 9 , wherein said increase in expression level of CIP2A in combination with said increase expression level of said cervical cancer biomarker provides an assay sensitivity of at least 90%.
11. The method of claim 9 , wherein said cervical cancer biomarker is Ki67.
12. The method of claim 9 , wherein said cervical cancer biomarker is TOP2A.
13. The method of claim 9 , wherein said cervical cancer biomarker is MCM2.
14. The method of claim 9 , wherein said cervical cancer biomarker is MCM5.
15. The method of claim 9 , wherein said cervical cancer biomarker is p14 ARF .
16. The method of claim 9 , wherein said cervical cancer biomarker is p16 INK4a .