Directional surface plasmon coupled fluorescence and chemiluminescence from thin films of nickel, iron or palladium and uses thereof
Nickel, iron and palladium thin films thermally evaporated onto glass supports are used to demonstrate surface plasmon coupled fluorescence (SPCF) and surface plasmon couple chemiluminescence (SPCC) over a broad wavelength range (400-800 nm) for potential assays or other detection systems. Nickel, iron and palladium thin films used in SPCF and SPCC convert otherwise isotropic emission into highly directional and polarized emission, an attractive concept for surface assays. The emission angles of detected emissions occur over a 10 degree range for tested emitted wavelengths.
1. A method of metal-enhanced luminescence sensing, comprising:
applying a continuous metallic thin film consisting of Ni to a substrate used in a detection system, wherein the substrate is glass, quartz, cellulose, polymeric material or a combination thereof and the metallic thin film consisting of Ni is immobilized on the surface and is from 15 nm to 20 nm thick;
introducing at least one excitable molecule for disposing near the metallic thin film, wherein the excitable molecule is capable of a chemically induced excited state or radiatively excited state and wherein the excitable molecule is positioned in a range from about 4 nm to 20 nm to the metallic thin film;
triggering the chemically induced excited state or radiatively excited state of the excitable molecule; and
detecting directional emissions for measuring emitted luminescent intensity, wherein the directional emissions are within a fixed 10 degree wide observation angle, located between 60 and 70 degrees from the normal of the surface.
2. The method of claim 1 , wherein the luminescence is chemiluminescence, bioluminescence or fluorescence.
3. The method of claim 1 , wherein the excitable molecule that is radiatively excited is a fluorophore.
4. The method of claim 3 , wherein the fluorophore has an emission wavelength in the range of about 400 to 900 nm.
5. The method of claim 1 , wherein the detected directional emissions are p-polarized emissions.
6. The method according to claim 3 , wherein the metallic thin film of Ni has attached thereto a captured biomolecular probe with an affinity for a target molecule, and the method further comprises:
contacting the captured biomolecular probe with a sample containing the target molecule wherein the target molecule binds to the captured biomolecular probe;
introducing a free biomolecular probe with an affinity for the target molecule, wherein the free biomolecular probe has attached thereto the fluorophore, wherein binding of the free biomolecular probe to the target molecule causes the fluorophore to be positioned a sufficient distance from the thin metal film of Ni to enhance fluorescence emission when excited by an irradiating source.