Agent for inhibiting production of hepatitis C virus and its use
This invention provides an agent for inhibiting production of hepatitis C virus with notable anti-HCV activity and without side-effects. The agent comprises a proanthocyanidin polymer composition illustrated in the following the general formula (1), wherein R 1 is hydrogen or hydroxyl, R 2 is hydroxyl, R 3 is hydrogen when R 1 is either hydrogen or hydroxyl, but R 3 is possibly hydroxyl when R 1 is either hydrogen or hydroxyl to the extent that both R 1 and R 3 being hydroxyl is at most 40 percents in the proanthocyanidin polymer composition said units of flavan-3-ol being bonded each other in any one of three patterns as follows; (i) a bond between carbon at the position 4 and carbon at the position 8, (ii) a bond between carbon at the position 4 and carbon at the position 6, (iii) a bond between carbon at the position 4 and carbon at the position 8, and between carbon, at the position 2 and oxygen at the position 7.
1. A method for producing a composition for inhibiting production of hepatitis C virus (HCV), comprising:
preparing a proanthocyanidin composition
having an anti-HCV production activity value (IC50) which is:
less than 0.61 μg/ml, and
less than or equal to one tenth of the concentration of the proanthocyanidin composition for inhibiting 50% of cell proliferation (CC50); and
comprising a proanthocyanidin having 5 to 10 monomer units, each of which consists of the flavan-3-ol illustrated in the general formula (1):
wherein R 1 is hydrogen, R 2 is hydroxyl, R 3 is hydrogen or hydroxyl, R 4 is hydrogen or a gallate group, and n is 5-10;
said units of flavan-3-ol being bonded to each other in any one of three bond patterns as follows:
(i) the bond between carbon at position 4 and carbon at position 8,
(ii) the bond between carbon at position 4 and carbon at position 6,
(iii) the bond between carbon at position 4 and carbon at position 8, and between carbon at position 2 and oxygen at position 7; and
forming the composition for inhibiting production of HCV comprising the proanthocyanidin composition.
2. The method according to claim 1 , wherein the proanthocyanidin composition is prepared from an extract of blueberry leaves.
3. The method according to claim 1 , wherein the mean polymerization degree of the proanthocyanidin is 5 to 9.
4. A method for inhibiting production of HCV comprising:
administering a composition prepared by the method according to claim 1 to a patient in need thereof.
5. The method according to claim 4 , wherein the proanthocyanidin composition is prepared from an extract of blueberry leaves.
6. The method according to claim 4 , wherein the mean polymerization degree of the proanthocyanidin is 5 to 9.