Microbial nanowires
View Patent ↗The application describes electrically conductive nanowires, as well as genetically and/or chemically modified nanowires with modified conductive, adhesive and/or coupling properties.
1. An isolated nucleic acid encoding a mutated nanowire polypeptide comprising the amino acid sequence of SEQ ID NO: 9 or 10, wherein at least one hydrophobic or apolar amino acid selected from the group consisting of A11, I12, I13, I15, L16, A17, A18, I19, A20, I21, P22, A26, V29, A31, A35, A36, 140, L43, A46, L47, A50, A52, P58 and P59 is substituted or replaced with a hydrophilic or aromatic amino acid to modify electrical conductivity.
2. The isolated nucleic acid of claim 1 , which is operably linked to an expression control sequence.
3. The isolated nucleic acid of claim 1 , which is incorporated into a replication or expression vector.
4. The isolated nucleic acid of claim 3 , wherein the vector further comprises a polyadenylation or transcriptional termination sequence.
5. An isolated host cell comprising the isolated nucleic acid of claim 1 or a replication or expression vector comprising said nucleic acid.
6. The isolated host cell of claim 5 , comprising a pilin depolymerase gene and/or a pilin polymerase gene from a Geobacteraceae bacterium.
7. The isolated host cell of claim 5 , wherein the cell is a prokaryotic or eukaryotic cell.
8. The isolated host cell of claim 5 , wherein the cell is a Gram-negative bacterium.
9. The isolated host cell of claim 5 , wherein the cell is a Geobacteraceae bacterium.
10. The isolated host cell of claim 5 , wherein the host cell does not have a pilin depolymerase gene.
11. The isolated nucleic acid of claim 1 , wherein the encoded polypeptide has at least one amino acid replaced with a tyrosine or tryptophan residue.
12. The isolated nucleic acid of claim 1 , wherein the encoded polypeptide has at least one amino acid replaced with an aspartic acid, glutamic acid, lysine or arginine residue.
13. The isolated nucleic acid of claim 1 , wherein the encoded polypeptide has at least 50% of the polypeptide's secondary structure as α-helix.
14. The isolated nucleic acid of claim 1 , wherein the encoded polypeptide assembles into an electrically conductive pilus.